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Mouse Delta-like protein 4 (DLL4) ELISA Kit

Mouse Delta-like protein 4 (DLL4) ELISA Kit

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Specification

Product name Mouse Delta-like protein 4 (DLL4) ELISA Kit
Reactivity Mouse
Applications ELISA
Applications notes This Mouse Delta-like protein 4 (DLL4) ELISA Kit employs a two-site sandwich ELISA to quantitate DLL4 in samples. An antibody specific for DLL4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDLL4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DLL4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DLL4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative DLL4; MGC126344; hdelta2; delta 4; delta ligand 4; delta-like 4 homolog; delta-like 4 protein; notch ligand DLL4; notch ligand delta-2

Product Properties

Kit components • Mouse Delta-like protein 4 microplate
• Mouse Delta-like protein 4 standard
• Mouse Delta-like protein 4 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Mouse Delta-like protein 4 (DLL4) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse DLL4. No significant cross-reactivity or interference between Mouse DLL4 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background DLL4 is a homolog of the Drosophila delta gene. The delta gene family encodes Notch ligands that are characterized by a DSL domain, EGF repeats, and a transmembrane domain. The predicted 685-amino acid DLL4 protein exhibits all of the hallmarks of the Delta family of Notch ligands. It has an extracellular region containing 8 EGF-like repeats and a DSL domain involved in Notch binding, as well as a transmembrane domain and a cytoplasmic tail that lacks catalytic motifs. Human DLL4 shares 87% amino acid sequence identity with mouse Dll4. Northern blot analysis of mouse tissues detected highest Dll4 expression in lung, followed by heart, kidney, skeletal muscle, and brain; Dll4 expression was barely detectable in spleen and testis.
Gene ID 54485
Alternative DLL4; MGC126344; hdelta2; delta 4; delta ligand 4; delta-like 4 homolog; delta-like 4 protein; notch ligand DLL4; notch ligand delta-2
Accession Q9JI71

Image & description

Fig.1. Mouse Delta-like protein 4 (DLL4) Standard Curve.

Fig.1. Mouse Delta-like protein 4 (DLL4) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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