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Mouse Delta-like protein 1 (DLL1) ELISA Kit

Mouse Delta-like protein 1 (DLL1) ELISA Kit

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Specification

Product name Mouse Delta-like protein 1 (DLL1) ELISA Kit
Reactivity Mouse
Applications ELISA
Applications notes This Mouse Delta-like protein 1 (DLL1) ELISA Kit employs a two-site sandwich ELISA to quantitate DLL1 in samples. An antibody specific for DLL1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDLL1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DLL1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DLL1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative DLL1; DELTA1; DL1; Delta; delta-like 1; delta-like 1 protein

Product Properties

Kit components • Mouse Delta-like protein 1 microplate
• Mouse Delta-like protein 1 standard
• Mouse Delta-like protein 1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Mouse Delta-like protein 1 (DLL1) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse DLL1. No significant cross-reactivity or interference between Mouse DLL1 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background DLL1 is a human homolog of the Notch Delta ligand and is a member of the delta/serrate/jagged family. It plays a role in mediating cell fate decisions during hematopoiesis. It may play a role in cell-to-cell communication. Sequence analysis predicted that the 723-amino acid DLL1 transmembrane protein, which is 88% identical to the mouse Dll1 protein, has a DSL domain followed by 8 tandem EGF-like repeats and a short cytoplasmic C-terminal region. Northern blot analysis revealed strongest expression of 4.0- and 4.6-kb transcripts in heart and pancreas, with lower expression in brain and muscle and almost no expression in placenta, lung, liver, and kidney. In situ hybridization analysis indicated upregulated expression of DLL1 in squamous cell carcinoma and in situ and invasive adenocarcinoma.
Gene ID 13388
Alternative DLL1; DELTA1; DL1; Delta; delta-like 1; delta-like 1 protein
Accession Q61483

Image & description

Fig.1. Mouse Delta-like protein 1 (DLL1) Standard Curve.

Fig.1. Mouse Delta-like protein 1 (DLL1) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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