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Mouse Delphilin (GRID2IP) ELISA Kit

Mouse Delphilin (GRID2IP) ELISA Kit

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Specification

Product name Mouse Delphilin (GRID2IP) ELISA Kit
Reactivity Mouse
Applications ELISA
Applications notes This Mouse Delphilin (GRID2IP) ELISA Kit employs a two-site sandwich ELISA to quantitate GRID2IP in samples. An antibody specific for GRID2IP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyGRID2IP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GRID2IP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GRID2IP bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative GRID2IP; Delphilin; glutamate receptor; ionotropic; delta 2 (Grid2) interacting protein 1

Product Properties

Kit components • Mouse Delphilin microplate
• Mouse Delphilin standard
• Mouse Delphilin detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Mouse Delphilin (GRID2IP) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse GRID2IP. No significant cross-reactivity or interference between Mouse GRID2IP and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Glutamate receptor delta-2 (GRID2) is predominantly expressed at parallel fiber-Purkinje cell postsynapses and plays crucial roles in synaptogenesis and synaptic plasticity. GRID2IP1 interacts with GRID2 and may control GRID2 signaling in Purkinje cells. The L-delphilin isoform contains an N-terminal PDZ domain (PDZ1), followed by a linker region, a second PDZ domain (PDZ2), an FH domain, and a C-terminal coiled-coil domain. Compared with L-delphilin, S-delphilin lacks PDZ1 and the linker region, which are replaced with an N-terminal palmitoylation site. Northern blot analysis of mouse brain showed higher expression of S-delphilin in cerebral cortex than cerebellum, whereas L-delphilin showed higher expression in cerebellum.
Gene ID 170935
Alternative GRID2IP; Delphilin; glutamate receptor; ionotropic; delta 2 (Grid2) interacting protein 1
Accession Q0QWG9

Image & description

Fig.1. Mouse Delphilin (GRID2IP) Standard Curve.

Fig.1. Mouse Delphilin (GRID2IP) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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