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Mouse Deleted in lung and esophageal cancer protein 1 (DLEC1) ELISA Kit

Mouse Deleted in lung and esophageal cancer protein 1 (DLEC1) ELISA Kit

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Specification

Product name Mouse Deleted in lung and esophageal cancer protein 1 (DLEC1) ELISA Kit
Reactivity Mouse
Applications ELISA
Applications notes This Mouse Deleted in lung and esophageal cancer protein 1 (DLEC1) ELISA Kit employs a two-site sandwich ELISA to quantitate DLEC1 in samples. An antibody specific for DLEC1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDLEC1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DLEC1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DLEC1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative DLEC1; DLC1; F56; deleted in lung cancer 1

Product Properties

Kit components • Mouse Deleted in lung and esophageal cancer protein 1 microplate
• Mouse Deleted in lung and esophageal cancer protein 1 standard
• Mouse Deleted in lung and esophageal cancer protein 1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Mouse Deleted in lung and esophageal cancer protein 1 (DLEC1) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse DLEC1. No significant cross-reactivity or interference between Mouse DLEC1 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background DLC1 contains 37 exons and spans approximately 59 kb. The predicted DLC1 protein contains 1,755 amino acids. Expression of DLC1 significantly suppressed the growth of 4 different cancer cell lines. Northern blot analysis revealed that DLC1 was expressed as 6- and 8-kb mRNAs in all tissues tested, most abundantly in prostate and testis. the 8-kb mRNA and several other alternatively spliced DLC1 transcripts contain disrupted coding regions and are likely to encode nonfunctional proteins. RT-PCR experiments indicated that 33% of primary lung, esophageal, and renal cancers and cancer cell lines tested either lacked DLC1 transcripts entirely or contained increased levels of nonfunctional DLC1 mRNA. The authors concluded that aberrant transcription of DLC1 may be involved in carcinogenesis of the lung, esophagus, and kidney.
Alternative DLEC1; DLC1; F56; deleted in lung cancer 1
Accession Q8BLA1

Image & description

Fig.1. Mouse Deleted in lung and esophageal cancer protein 1 (DLEC1) Standard Curve.

Fig.1. Mouse Deleted in lung and esophageal cancer protein 1 (DLEC1) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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