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Mouse Dapper homolog 1 (DACT1) ELISA Kit

Mouse Dapper homolog 1 (DACT1) ELISA Kit

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Specification

Product name Mouse Dapper homolog 1 (DACT1) ELISA Kit
Reactivity Mouse
Applications ELISA
Applications notes This Mouse Dapper homolog 1 (DACT1) ELISA Kit employs a two-site sandwich ELISA to quantitate DACT1 in samples. An antibody specific for DACT1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDACT1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DACT1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DACT1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative DACT1; DAPPER; DAPPER1; DPR1; FRODO; HDPR1; THYEX3; dapper 1; dapper homolog 1; antagonist of beta-catenin; heptacellular carcinoma novel gene 3

Product Properties

Kit components • Mouse Dapper homolog 1 microplate
• Mouse Dapper homolog 1 standard
• Mouse Dapper homolog 1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Mouse Dapper homolog 1 (DACT1) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse DACT1. No significant cross-reactivity or interference between Mouse DACT1 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background The protein encoded by DACT1 belongs to the dapper family, characterized by the presence of PDZ-binding motif at the C-terminus. It interacts with, and positively regulates dishevelled-mediated signaling pathways during development. Depletion of this mRNA from xenopus embryos resulted in loss of notochord and head structures, and mice lacking this gene died shortly after birth from severe posterior malformations. Alternatively spliced transcript variants have been found for this gene.
Gene ID 59036
Alternative DACT1; DAPPER; DAPPER1; DPR1; FRODO; HDPR1; THYEX3; dapper 1; dapper homolog 1; antagonist of beta-catenin; heptacellular carcinoma novel gene 3
Accession Q8R4A3

Image & description

Fig.1. Mouse Dapper homolog 1 (DACT1) Standard Curve.

Fig.1. Mouse Dapper homolog 1 (DACT1) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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