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Mouse Dachshund homolog 1 (DACH1) ELISA Kit

Mouse Dachshund homolog 1 (DACH1) ELISA Kit

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Specification

Product name Mouse Dachshund homolog 1 (DACH1) ELISA Kit
Reactivity Mouse
Applications ELISA
Applications notes This Mouse Dachshund homolog 1 (DACH1) ELISA Kit employs a two-site sandwich ELISA to quantitate DACH1 in samples. An antibody specific for DACH1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDACH1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DACH1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DACH1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative DACH1; RP11-512J14.1; DACH; FLJ10138; dachshund homolog 1

Product Properties

Kit components • Mouse Dachshund homolog 1 microplate
• Mouse Dachshund homolog 1 standard
• Mouse Dachshund homolog 1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Mouse Dachshund homolog 1 (DACH1) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse DACH1. No significant cross-reactivity or interference between Mouse DACH1 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background DACH1 encodes a chromatin-associated protein that associates with other DNA-binding transcription factors to regulate gene expression and cell fate determination during development. The protein contains a Ski domain that is highly conserved from Drosophila to human. Expression of this gene is lost in some forms of metastatic cancer, and is correlated with poor prognosis. Multiple transcript variants encoding different isoforms have been found for this gene.
Gene ID 13134
Alternative DACH1; RP11-512J14.1; DACH; FLJ10138; dachshund homolog 1
Accession Q9QYB2

Image & description

Fig.1. Mouse Dachshund homolog 1 (DACH1) Standard Curve.

Fig.1. Mouse Dachshund homolog 1 (DACH1) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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