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Mouse Cytosolic Fe-S cluster assembly factor NUBP2 (NUBP2) ELISA Kit

Mouse Cytosolic Fe-S cluster assembly factor NUBP2 (NUBP2) ELISA Kit

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Specification

Product name Mouse Cytosolic Fe-S cluster assembly factor NUBP2 (NUBP2) ELISA Kit
Reactivity Mouse
Applications ELISA
Applications notes This Mouse Cytosolic Fe-S cluster assembly factor NUBP2 (NUBP2) ELISA Kit employs a two-site sandwich ELISA to quantitate NUBP2 in samples. An antibody specific for NUBP2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyNUBP2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NUBP2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NUBP2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative NUBP2; CFD1; NUBP1; C447E6.1 (nucleotide binding protein 1 (E.coli MinD like) ); homolog of yeast cytosolic Fe-S cluster deficient 1; nucleotide binding protein 2 (E.coli MinD like)

Product Properties

Kit components • Mouse Cytosolic Fe-S cluster assembly factor NUBP2 microplate
• Mouse Cytosolic Fe-S cluster assembly factor NUBP2 standard
• Mouse Cytosolic Fe-S cluster assembly factor NUBP2 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Mouse Cytosolic Fe-S cluster assembly factor NUBP2 (NUBP2) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse NUBP2. No significant cross-reactivity or interference between Mouse NUBP2 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background NUBP2 is a member of the NUBP/MRP gene subfamily of ATP-binding proteins. Nakashima et al. (1999) cloned mouse Nubp2 and used the murine sequence as a probe in EST database analysis, followed by screening a human teratoma cDNA library to clone full-length human NUBP2. The 271-amino acid human NUBP2 protein shares 72.6% amino acid similarity with mouse Nubp2. Northern blot analysis detected a 1.4-kb NUBP2 transcript with ubiquitous expression in all human adult and fetal tissues tested, with highest expression in adult skeletal muscle. NUBP2 contains conserved an ATP/GTP binding motif A (P-loop), an ATP/GTP binding motif A-prime, and NUBP/MRP alpha and beta motifs. NUBP2 lacks an additional N-terminal sequence with 4 cysteine residues that is present in NUBP1.
Gene ID 26426
Alternative NUBP2; CFD1; NUBP1; C447E6.1 (nucleotide binding protein 1 (E.coli MinD like) ); homolog of yeast cytosolic Fe-S cluster deficient 1; nucleotide binding protein 2 (E.coli MinD like)
Accession Q9R061

Image & description

Fig.1. Mouse Cytosolic Fe-S cluster assembly factor NUBP2 (NUBP2) Standard Curve.

Fig.1. Mouse Cytosolic Fe-S cluster assembly factor NUBP2 (NUBP2) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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