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Mouse Cytochrome b5 type B (CYB5B) ELISA Kit

Mouse Cytochrome b5 type B (CYB5B) ELISA Kit

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Specification

Product name Mouse Cytochrome b5 type B (CYB5B) ELISA Kit
Reactivity Mouse
Applications ELISA
Applications notes This Mouse Cytochrome b5 type B (CYB5B) ELISA Kit employs a two-site sandwich ELISA to quantitate CYB5B in samples. An antibody specific for CYB5B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyCYB5B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CYB5B is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CYB5B bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative CYB5B; CYB5-M; CYPB5M; DKFZp686M0619; OMB5; cytochrome b5 outer mitochondrial membrane; outer mitochondrial membrane cytochrome b5; type 2 cyt-b5

Product Properties

Kit components • Mouse Cytochrome b5 type B microplate
• Mouse Cytochrome b5 type B standard
• Mouse Cytochrome b5 type B detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Mouse Cytochrome b5 type B (CYB5B) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse CYB5B. No significant cross-reactivity or interference between Mouse CYB5B and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background CYB5B (Cytochrome B5 Type B) is a Protein Coding gene. Among its related pathways are Metabolism and Cytochrome P450 - arranged by substrate type. GO annotations related to this gene include heme binding and enzyme activator activity. An important paralog of this gene is CYB5A. Cytochrome b5 is a membrane bound hemoprotein which function as an electron carrier for several membrane bound oxygenases.
Gene ID 66427
Alternative CYB5B; CYB5-M; CYPB5M; DKFZp686M0619; OMB5; cytochrome b5 outer mitochondrial membrane; outer mitochondrial membrane cytochrome b5; type 2 cyt-b5
Accession Q9CQX2

Image & description

Fig.1. Mouse Cytochrome b5 type B (CYB5B) Standard Curve.

Fig.1. Mouse Cytochrome b5 type B (CYB5B) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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