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Mouse Collagen alpha-1 (III) chain (COL3A1) ELISA Kit

Mouse Collagen alpha-1 (III) chain (COL3A1) ELISA Kit

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Specification

Product name Mouse Collagen alpha-1 (III) chain (COL3A1) ELISA Kit
Reactivity Mouse
Applications ELISA
Applications notes This Mouse Collagen alpha-1 (III) chain (COL3A1) ELISA Kit employs a two-site sandwich ELISA to quantitate COL3A1 in samples. An antibody specific for COL3A1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyCOL3A1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for COL3A1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of COL3A1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative COL3A1; EDS4A; FLJ34534; Ehlers-Danlos syndrome type IV; autosomal dominant; alpha1 (III) collagen; collagen typeI alpha 1; collagen; fetal

Product Properties

Kit components • Mouse Collagen alpha-1 microplate
• Mouse Collagen alpha-1 standard
• Mouse Collagen alpha-1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Mouse Collagen alpha-1 (III) chain (COL3A1) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse COL3A1. No significant cross-reactivity or interference between Mouse COL3A1 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Collagen alpha-1(III) chain is a fibrillar collagen that is found in extensible connective tissues such as skin, lung, and the vascular system, frequently in association with type I collagen. Mutations in this gene are associated with Ehlers-Danlos syndrome type IV, and with aortic and arterial aneurysms. Although alternate transcripts have been detected for this gene, they are the result of mutations; these mutations alter splicing, often leading to the exclusion of multiple exons.By fluorescence in situ hybridization, Limongi et al. (1997) concluded that the COL3A1 gene is located in chromosome band 2q32.2 and that the nebulin gene is located in band 2q24.2; the FRA2G fragile site was found to lie between the 2 genes in the 2q31 band.
Gene ID 12825
Alternative COL3A1; EDS4A; FLJ34534; Ehlers-Danlos syndrome type IV; autosomal dominant; alpha1 (III) collagen; collagen typeI alpha 1; collagen; fetal
Accession P08121

Image & description

Fig.1. Mouse Collagen alpha-1 (III) chain (COL3A1) Standard Curve.

Fig.1. Mouse Collagen alpha-1 (III) chain (COL3A1) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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