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Mouse Chordin-like protein 2 (CHRDL2) ELISA Kit

Mouse Chordin-like protein 2 (CHRDL2) ELISA Kit

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Specification

Product name Mouse Chordin-like protein 2 (CHRDL2) ELISA Kit
Reactivity Mouse
Applications ELISA
Applications notes This Mouse Chordin-like protein 2 (CHRDL2) ELISA Kit employs a two-site sandwich ELISA to quantitate CHRDL2 in samples. An antibody specific for CHRDL2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyCHRDL2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CHRDL2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CHRDL2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative CHRDL2; BNF1; CHL2; DKFZp586N2124; FKSG37; breast tumor novel factor 1

Product Properties

Kit components • Mouse Chordin-like protein 2 microplate
• Mouse Chordin-like protein 2 standard
• Mouse Chordin-like protein 2 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Mouse Chordin-like protein 2 (CHRDL2) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse CHRDL2. No significant cross-reactivity or interference between Mouse CHRDL2 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background The deduced 429-amino acid human protein shares 73% homology with the 426-amino acid mouse protein. CHL2 contains an N-terminal signal peptide, followed by 3 chordin (CHRD)-like cysteine-rich repeats (CR1 through CR3) and a C-terminal tail. Northern blot analysis detected high expression of a 2.0-kb CHL2 transcript in adult human uterus, with lower expression in colon, heart, prostate, stomach, and skeletal muscle. Expression was weak in placenta, testis, small intestine, trachea, and bone marrow, and no expression was detected in liver or kidney. In situ hybridization of mouse embryos showed that Chl2 expression was restricted to chondrocytes of developing joint cartilage. In adult mice, Chl2 expression was detected in connective tissues of reproductive organs, with highest expression in uterine myometrium.
Gene ID 69121
Alternative CHRDL2; BNF1; CHL2; DKFZp586N2124; FKSG37; breast tumor novel factor 1
Accession Q8VEA6

Image & description

Fig.1. Mouse Chordin-like protein 2 (CHRDL2) Standard Curve.

Fig.1. Mouse Chordin-like protein 2 (CHRDL2) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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