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Mouse Cerberus (CER1) ELISA Kit

Mouse Cerberus (CER1) ELISA Kit

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Specification

Product name Mouse Cerberus (CER1) ELISA Kit
Reactivity Mouse
Applications ELISA
Applications notes This Mouse Cerberus (CER1) ELISA Kit employs a two-site sandwich ELISA to quantitate CER1 in samples. An antibody specific for CER1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyCER1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CER1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CER1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative CER1; DAND4; MGC119894; MGC119895; MGC96951; cerberus 1; cerberus-related 1

Product Properties

Kit components • Mouse Cerberus microplate
• Mouse Cerberus standard
• Mouse Cerberus detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Mouse Cerberus (CER1) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse CER1. No significant cross-reactivity or interference between Mouse CER1 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Cerberus also known as CER1 is a cytokine member of the cystine knot superfamily, characterized by nine conserved cysteines and a cysteine knot region. The cerberus-related cytokines, together with Dan and DRM / Gremlin, represent a group of bone morphogenetic protein (BMP) antagonists that can bind directly to BMPs and inhibit their activity.Cerberus is an inhibitor in the TGF beta signaling pathway secreted during the gastrulation phase of the embryogenesis.Cer1 mimicked the anti-BMP activity of Xcer in Xenopus animal caps, although with reduced potency. In situ hybridization to early gastrula mouse embryos revealed that Cer1 expression was restricted to the anterior primitive endoderm, the tissue thought to initiate head induction.
Gene ID 12622
Alternative CER1; DAND4; MGC119894; MGC119895; MGC96951; cerberus 1; cerberus-related 1
Accession O55233

Image & description

Fig.1. Mouse Cerberus (CER1) Standard Curve.

Fig.1. Mouse Cerberus (CER1) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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