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Mouse CCAAT/enhancer-binding protein beta (CEBPB) ELISA Kit

Mouse CCAAT/enhancer-binding protein beta (CEBPB) ELISA Kit

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Specification

Product name Mouse CCAAT/enhancer-binding protein beta (CEBPB) ELISA Kit
Reactivity Mouse
Applications ELISA
Applications notes This Mouse CCAAT/enhancer-binding protein beta (CEBPB) ELISA Kit employs a two-site sandwich ELISA to quantitate CEBPB in samples. An antibody specific for CEBPB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyCEBPB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CEBPB is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CEBPB bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative CEBPB; C/EBP-beta; CRP2; IL6DBP; LAP; MGC32080; NF-IL6; TCF5; CCAAT/enhancer binding protein beta; interleukin 6-dependent DNA-binding protein; liver-enriched transcriptional activator protein; nuclear factor

Product Properties

Kit components • Mouse CCAAT/enhancer-binding protein beta microplate
• Mouse CCAAT/enhancer-binding protein beta standard
• Mouse CCAAT/enhancer-binding protein beta detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Mouse CCAAT/enhancer-binding protein beta (CEBPB) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse CEBPB. No significant cross-reactivity or interference between Mouse CEBPB and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background CCAAT/enhancer-binding protein beta is aencoded by this intronless gene is a bZIP transcription factor which can bind as a homodimer to certain DNA regulatory regions. It can also form heterodimers with the related proteins CEBP-alpha, CEBP-delta, and CEBP-gamma.CEBPbis important in the regulation of genes involved in immune and inflammatory responses and has been shown to bind to the IL-1 response element in the IL-6 gene, as well as to regulatory regions of several acute-phase and cytokine genes. In addition, the encoded protein can bind the promoter and upstream element and stimulate the expression of the collagen type I gene.
Gene ID 12608
Alternative CEBPB; C/EBP-beta; CRP2; IL6DBP; LAP; MGC32080; NF-IL6; TCF5; CCAAT/enhancer binding protein beta; interleukin 6-dependent DNA-binding protein; liver-enriched transcriptional activator protein; nuclear factor
Accession P28033

Image & description

Fig.1. Mouse CCAAT/enhancer-binding protein beta (CEBPB) Standard Curve.

Fig.1. Mouse CCAAT/enhancer-binding protein beta (CEBPB) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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