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Mouse Calpastatin (CAST) ELISA Kit

Mouse Calpastatin (CAST) ELISA Kit

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Specification

Product name Mouse Calpastatin (CAST) ELISA Kit
Reactivity Mouse
Applications ELISA
Applications notes This Mouse Calpastatin (CAST) ELISA Kit employs a two-site sandwich ELISA to quantitate CAST in samples. An antibody specific for CAST has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyCAST present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CAST is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CAST bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative CAST; BS-17; MGC9402; OTTHUMP00000158520; calpain inhibitor; heart-type calpastatin; sperm BS-17 component

Product Properties

Kit components • Mouse Calpastatin microplate
• Mouse Calpastatin standard
• Mouse Calpastatin detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Mouse Calpastatin (CAST) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse CAST. No significant cross-reactivity or interference between Mouse CAST and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Calpastatin is an endogenous calpain (calcium-dependent cysteine protease) inhibitor. It consists of an N-terminal domain L and four repetitive calpain-inhibition domains (domains 1-7), and it is involved in the proteolysis of amyloid precursor protein. T愼愼愼
Gene ID 12380
Alternative CAST; BS-17; MGC9402; OTTHUMP00000158520; calpain inhibitor; heart-type calpastatin; sperm BS-17 component
Accession P51125

Image & description

Fig.1. Mouse Calpastatin (CAST) Standard Curve.

Fig.1. Mouse Calpastatin (CAST) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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