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Mouse Bone morphogenetic protein 6 (BMP-6) ELISA Kit

Mouse Bone morphogenetic protein 6 (BMP-6) ELISA Kit

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Specification

Product name Mouse Bone morphogenetic protein 6 (BMP-6) ELISA Kit
Reactivity Mouse
Applications ELISA
Applications notes This Mouse Bone morphogenetic protein 6 (BMP-6) ELISA Kit employs a two-site sandwich ELISA to quantitate BMP6 in samples. An antibody specific for BMP6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyBMP6 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for BMP6 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of BMP6 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative BMP6; VGR; VGR1; Vg1-related sequence; vegetal related growth factor (TGFB-related); vegetal-related (TGFB related) cytokine

Product Properties

Kit components • Mouse Bone morphogenetic protein 6 microplate
• Mouse Bone morphogenetic protein 6 standard
• Mouse Bone morphogenetic protein 6 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Mouse Bone morphogenetic protein 6 (BMP-6) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse BMP6. No significant cross-reactivity or interference between Mouse BMP6 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Bone morphogenetic proteins, such as BMP6, belong to the transforming growth factor-beta superfamily of regulatory molecules (Rickard et al., 1998).Rickard et al. (1998) presented evidence that the skeletal effects of estrogen on bone and cartilage may be mediated by increased production of BMP6 by osteoblasts. They investigated the effect of estrogen on BMP production in 2 estrogen-responsive, human immortalized cell lines that display the mature osteoblast phenotype.Tamada et al. (1998) found that the promoter region of the BMP6 gene lacks a canonical TATA box, but has a GC-rich region with 2 SP1-binding sites, an inverted CCAAT element, and a putative tramtrack responsive element. Reporter gene assays suggested that the BMP6 promoter has osteogenic cell specificity.
Gene ID 12161
Alternative BMP6; VGR; VGR1; Vg1-related sequence; vegetal related growth factor (TGFB-related); vegetal-related (TGFB related) cytokine
Accession P20722

Image & description

Fig.1. Mouse Bone morphogenetic protein 6 (BMP-6) Standard Curve.

Fig.1. Mouse Bone morphogenetic protein 6 (BMP-6) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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