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Mouse Bile salt export pump (ABCB11) ELISA Kit

Mouse Bile salt export pump (ABCB11) ELISA Kit

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Specification

Product name Mouse Bile salt export pump (ABCB11) ELISA Kit
Reactivity Mouse
Applications ELISA
Applications notes This Mouse Bile salt export pump (ABCB11) ELISA Kit employs a two-site sandwich ELISA to quantitate ABCB11 in samples. An antibody specific for ABCB11 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyABCB11 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ABCB11 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ABCB11 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative ABCB11; ABC16; BRIC2; BSEP; PFIC-2; PFIC2; PGY4; SPGP; ABC member 16; MDR/TAP subfamily; bile salt export pump; progressive familial intrahepatic cholestasis 2; sister p-glycoprotein

Product Properties

Kit components • Mouse Bile salt export pump microplate
• Mouse Bile salt export pump standard
• Mouse Bile salt export pump detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Mouse Bile salt export pump (ABCB11) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse ABCB11. No significant cross-reactivity or interference between Mouse ABCB11 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background ABCB11 is an ABC transporter named BSEP (Bile Salt Export Pump), or sPgp (sister of P-glycoprotein). This membrane-associated protein is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White).This protein is a member of the MDR/TAP subfamily. Some members of the MDR/TAP subfamily are involved in multidrug resistance. This particular protein is responsible for the transport of taurocholate and other cholate conjugates from hepatocytes (liver cells) to the bile. In humans, the activity of this transporter is the major determinant of bile formation and bile flow.
Gene ID 27413
Alternative ABCB11; ABC16; BRIC2; BSEP; PFIC-2; PFIC2; PGY4; SPGP; ABC member 16; MDR/TAP subfamily; bile salt export pump; progressive familial intrahepatic cholestasis 2; sister p-glycoprotein
Accession Q9QY30

Image & description

Fig.1. Mouse Bile salt export pump (ABCB11) Standard Curve.

Fig.1. Mouse Bile salt export pump (ABCB11) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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