Product Information

• Product name

  Mouse BDNF Protein

• Sequence

  Amino acid sequence derived from mouse BDNF (P21237) (Met1-Arg249) was expressed and its mature form was purified. The mature form of human, mouse, rat, cynomolgus and canine BDNF sequences are identical.

• Activity

  Measured by its ability to bind biotinylated human TrkB-His in functional ELISA. Measured by its ability to bind Human TrkB-Fch in functional ELISA. Measured by its ability to bind biotinylated mouse TrkB-His in functional ELISA. 2. Measured by its ability to bind Human TrkB-Fch (Cat:10047-H03H) in functional ELISA. 3. Measured by its ability to bind biotinylated mouse TrkB-His (Cat:50132-M08H) in functional ELISA.

• Protein length

  The recombinant mature form of mouse BDNF consists of 119 amino acids and predicts a molecular mass of 13.5 KDa.

• Preparation method

  CHO Stable Cells

• Purity

  > 95 % as determined by SDS-PAGE

Fig.SDS-PAGE analysis of Mouse BDNF protein.

Product Properties

• Formulation

  Lyophilized from sterile PBS

• Molecular weight

  13.5 kDa

• Usage notes

  Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Mouse BDNF protein in sterile ddH2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

• Storage instructions

  Lyophilized Mouse BDNF protein product should be stored desiccated below -20°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

• Shipping

  The product is shipped at ambient temperature.

• Precautions

  The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

• Background

  BDNF is a member of the nerve growth factor family. It is highly expressed in hippocampus, amygdala, cerebral cortex and cerebellum. It also can be detected in heart, lung, skeletal muscle, testis, prostate and placenta. BDNF is induced by cortical neurons, and is necessary for survival of striatal neurons in the brain. During development, BDNF promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. It participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. It functions as the major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability.

• Gene ID

  12064

• Alternative names

  Brain-derived neurotrophic factor; BDNF

• Others

  The Endotoxin level is less than 1.0 EU per μg of the protein as determined by the LAL method

• Accession

  P21237