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Mouse ATP-dependent RNA helicase DHX8 (DHX8) ELISA Kit

Mouse ATP-dependent RNA helicase DHX8 (DHX8) ELISA Kit

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Specification

Product name Mouse ATP-dependent RNA helicase DHX8 (DHX8) ELISA Kit
Reactivity Mouse
Applications ELISA
Applications notes This Mouse ATP-dependent RNA helicase DHX8 (DHX8) ELISA Kit employs a two-site sandwich ELISA to quantitate DHX8 in samples. An antibody specific for DHX8 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDHX8 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DHX8 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DHX8 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative DHX8; DDX8; HRH1; PRP22; PRPF22; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 8 (RNA helicase); DEAH-box protein 8

Product Properties

Kit components • Mouse ATP-dependent RNA helicase DHX8 microplate
• Mouse ATP-dependent RNA helicase DHX8 standard
• Mouse ATP-dependent RNA helicase DHX8 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Mouse ATP-dependent RNA helicase DHX8 (DHX8) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse DHX8. No significant cross-reactivity or interference between Mouse DHX8 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background DHX8 is a member of the DEAH box polypeptide family. The encoded protein contains the DEAH (Asp-Glu-Ala-His) motif which is characteristic of all DEAH box proteins, and is thought to function as an ATP-dependent RNA helicase that regulates the release of spliced mRNAs from spliceosomes prior to their export from the nucleus. This protein may be required for the replication of human immunodeficiency virus type 1 (HIV-1). Alternative splicing results in multiple transcript variants.
Gene ID 217207
Alternative DHX8; DDX8; HRH1; PRP22; PRPF22; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 8 (RNA helicase); DEAH-box protein 8
Accession A2A4P0

Image & description

Fig.1. Mouse ATP-dependent RNA helicase DHX8 (DHX8) Standard Curve.

Fig.1. Mouse ATP-dependent RNA helicase DHX8 (DHX8) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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