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Mouse ATP-binding cassette sub-family A member 13 (ABCA13) ELISA Kit

Mouse ATP-binding cassette sub-family A member 13 (ABCA13) ELISA Kit

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Specification

Product name Mouse ATP-binding cassette sub-family A member 13 (ABCA13) ELISA Kit
Reactivity Mouse
Applications ELISA
Applications notes This Mouse ATP-binding cassette sub-family A member 13 (ABCA13) ELISA Kit employs a two-site sandwich ELISA to quantitate ABCA13 in samples. An antibody specific for ABCA13 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyABCA13 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ABCA13 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ABCA13 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative ABCA13; DKFZp313D2411; FLJ16398; FLJ33876; FLJ33951; ATP binding cassette transporter A13; ATP binding cassette; sub-family A (ABC1); member 13

Product Properties

Kit components • Mouse ATP-binding cassette sub-family A member 13 microplate
• Mouse ATP-binding cassette sub-family A member 13 standard
• Mouse ATP-binding cassette sub-family A member 13 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Mouse ATP-binding cassette sub-family A member 13 (ABCA13) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse ABCA13. No significant cross-reactivity or interference between Mouse ABCA13 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background ABCA13 is a member of ABC gene subfamily A (ABCA). Genes within the ABCA family typically encode several thousand amino acids. Like other ABC transmembrane transporter proteins, this protein has 12 or more transmembrane alpha-helix domains that likely arrange to form a single central chamber with multiple substrate binding sites. It is also predicted to have two large extracellular domains and two nucleotide binding domains as is typical for ABCA proteins. Alternative splice variants have been described but their biological validity has not been demonstrated. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
Gene ID 268379
Alternative ABCA13; DKFZp313D2411; FLJ16398; FLJ33876; FLJ33951; ATP binding cassette transporter A13; ATP binding cassette; sub-family A (ABC1); member 13
Accession Q5SSE9

Image & description

Fig.1. Mouse ATP-binding cassette sub-family A member 13 (ABCA13) Standard Curve.

Fig.1. Mouse ATP-binding cassette sub-family A member 13 (ABCA13) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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