|Product name||Mouse Anti-Chlamydia trachomatis (CT-IgG) ELISA Kit|
|Applications notes||This Mouse Anti-Chlamydia trachomatis (CT-IgG) ELISA Kit employs a two-site sandwich ELISA to quantitate CT-IgG in samples. An antibody specific for CT-IgG has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyCT-IgG present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CT-IgG is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CT-IgG bound in the initial step. The color development is stopped and the intensity of the color is measured.|
|SampleType||Cell culture supernatants, Other biological fluids, Plasma, Serum|
|Assay type||Sandwich ELISA (quantitative)|
|Assay duration||Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.|
|Kit components||• Mouse Anti-Chlamydia trachomatis microplate
• Mouse Anti-Chlamydia trachomatis standard
• Mouse Anti-Chlamydia trachomatis detect antibody
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
|Features & Benefits||Mouse Anti-Chlamydia trachomatis (CT-IgG) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse CT-IgG. No significant cross-reactivity or interference between Mouse CT-IgG and analogues was observed.|
|Calibration range||Please inquire|
|Limit of detection||Please inquire|
|Usage notes||• Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
|Storage instructions||The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.|
|Shipping||Gel pack with blue ice.|
|Precautions||The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.|
|Background||Chlamydia trachomatis, commonly known as chlamydia, is one of four bacterial species in the genus Chlamydia. Chlamydia is a genus of pathogenic bacteria that are obligate intracellular parasites. C. trachomatis is a Gram-negative bacterium. It is ovoid in shape and nonmotile. The bacteria are nonspore-forming, but the elementary bodies act like spores when released into the host. The inclusion bodies of C. trachomatis were first described in 1907 by Stanislaus von Prowazek and Ludwig Halberstädter during research on trachoma. C. trachomatis agent was first cultured in the yolk sacs of eggs by Professor Tang Fei-fan, et al. in 1957.|
Fig.1. Mouse Anti-Chlamydia trachomatis (CT-IgG) Standard Curve.
Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.