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Mouse Alpha-Melanocyte Stimulating Hormone (A-MSH) ELISA Kit

Mouse Alpha-Melanocyte Stimulating Hormone (A-MSH) ELISA Kit

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Specification

Product name Mouse Alpha-Melanocyte Stimulating Hormone (A-MSH) ELISA Kit
Reactivity Mouse
Applications ELISA
Applications notes This Mouse Alpha-Melanocyte Stimulating Hormone (A-MSH) ELISA Kit employs a two-site sandwich ELISA to quantitate A-MSH in samples. An antibody specific for A-MSH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyA-MSH present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for A-MSH is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of A-MSH bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative A-MSH; α-MSH; Intermedins; Alpha-Melanotropin, Alpha-Melanocortin; Alpha-Intermedin

Product Properties

Kit components • Mouse Alpha-Melanocyte Stimulating Hormone microplate
• Mouse Alpha-Melanocyte Stimulating Hormone standard
• Mouse Alpha-Melanocyte Stimulating Hormone detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Mouse Alpha-Melanocyte Stimulating Hormone (A-MSH) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse A-MSH. No significant cross-reactivity or interference between Mouse A-MSH and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Melanocyte-stimulating hormone belongs to a group called the melanocortins. This group includes ACTH, alpha-MSH, beta-MSH and gamma-MSH; these peptides are all cleavage products of a large precursor peptide called pro-opiomelanocortin (POMC). Alpha-MSH is the most important melanocortin for pigmentation.Alpha-MSH could be a potent biological marker for the diagnosis of CFS. Despite extensive research, no reliable biological marker for chronic fatigue syndrome (CFS) has yet been identified. However, hyperactivation of melanotrophs in the pituitary gland and increased levels of plasma alpha-melanocyte-stimulating hormone (alpha-MSH) have recently been detected in an animal model of chronic stress. Because CFS is considered to be caused partly by chronic stress events, increased alpha-MSH plasma levels may also occur in CFS patients.
Alternative A-MSH; α-MSH; Intermedins; Alpha-Melanotropin, Alpha-Melanocortin; Alpha-Intermedin

Image & description

Fig.1. Mouse Alpha-Melanocyte Stimulating Hormone (A-MSH) Standard Curve.

Fig.1. Mouse Alpha-Melanocyte Stimulating Hormone (A-MSH) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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