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Mouse Actin, cytoplasmic 1 (ACTB) ELISA Kit

Mouse Actin, cytoplasmic 1 (ACTB) ELISA Kit

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Specification

Product name Mouse Actin, cytoplasmic 1 (ACTB) ELISA Kit
Reactivity Mouse
Applications ELISA
Applications notes This Mouse Actin, cytoplasmic 1 (ACTB) ELISA Kit employs a two-site sandwich ELISA to quantitate ACTB in samples. An antibody specific for ACTB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyACTB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ACTB is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ACTB bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative ACTB; PS1TP5BP1; PS1TP5-binding protein 1; actin; cytoplasmic 1; beta actin; beta cytoskeletal actin

Product Properties

Kit components • Mouse Actin, cytoplasmic 1 microplate
• Mouse Actin, cytoplasmic 1 standard
• Mouse Actin, cytoplasmic 1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Mouse Actin, cytoplasmic 1 (ACTB) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse ACTB. No significant cross-reactivity or interference between Mouse ACTB and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Beta-actin (gene name ACTB) is one of the two nonmuscle cytoskeletal actins. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Alpha actins are a major constituent of the contractile apparatus. In a neoplastic cell line resulting from treatment of cultured human diploid fibroblasts with a chemical mutagen, Leavitt et al. (1982) observed a mutant form of beta actin. Toyama and Toyama (1984) isolated and characterized lines of KB cells resistant to cytochalasin B. They found that one resistant line had an alteration in beta-actin. Such cells bound less cytochalasin B than did parental KB cells. The authors suggested that the primary site of action of cytochalasin B on cell motility processes is beta-actin.
Gene ID 11461
Alternative ACTB; PS1TP5BP1; PS1TP5-binding protein 1; actin; cytoplasmic 1; beta actin; beta cytoskeletal actin
Accession P60710

Image & description

Fig.1. Mouse Actin, cytoplasmic 1 (ACTB) Standard Curve.

Fig.1. Mouse Actin, cytoplasmic 1 (ACTB) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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