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Mouse 52 kDa repressor of the inhibitor of the protein kinase (PRKRIR) ELISA Kit

Mouse 52 kDa repressor of the inhibitor of the protein kinase (PRKRIR) ELISA Kit

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Specification

Product name Mouse 52 kDa repressor of the inhibitor of the protein kinase (PRKRIR) ELISA Kit
Reactivity Mouse
Applications ELISA
Applications notes This Mouse 52 kDa repressor of the inhibitor of the protein kinase (PRKRIR) ELISA Kit employs a two-site sandwich ELISA to quantitate PRKRIR in samples. An antibody specific for PRKRIR has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPRKRIR present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRKRIR is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRKRIR bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative PRKRIR; DAP4; MGC102750; P52rIPK; THAP0; death-associated protein 4; inhibitor of protein kinase PKR

Product Properties

Kit components • Mouse 52 kDa repressor of the inhibitor of the protein kinase microplate
• Mouse 52 kDa repressor of the inhibitor of the protein kinase standard
• Mouse 52 kDa repressor of the inhibitor of the protein kinase detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Mouse 52 kDa repressor of the inhibitor of the protein kinase (PRKRIR) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse PRKRIR. No significant cross-reactivity or interference between Mouse PRKRIR and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background PRKRIR contains a region (amino acids 86 to 200) of limited homology (24% identity) to the charged domain of HSP90.The THAP domain of the deduced 761-amino acid THAP0 protein includes a C2CH signature, an AVPTIF box, and several other conserved amino acids. When coexpressed with PKR in yeast, P58(IPK) repressed PKR-mediated EIF2-alpha phosphorylation, inhibiting the normally toxic and growth-suppressive effects associated with PKR function. Conversely, introduction of P52(rIPK) into these strains resulted in restoration of both PKR activity and EIF2-alpha phosphorylation, concomitant with growth suppression due to inhibition of P58(IPK)
Gene ID 72981
Alternative PRKRIR; DAP4; MGC102750; P52rIPK; THAP0; death-associated protein 4; inhibitor of protein kinase PKR
Accession Q9CUX1

Image & description

Fig.1. Mouse 52 kDa repressor of the inhibitor of the protein kinase (PRKRIR) Standard Curve.

Fig.1. Mouse 52 kDa repressor of the inhibitor of the protein kinase (PRKRIR) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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