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MKP-1/2 Polyclonal Antibody

MKP-1/2 Polyclonal Antibody

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Specification

Product name MKP-1/2 Polyclonal Antibody
Immunogen Synthesized peptide derived from human MKP-1/2 around the non-phosphorylation site of S296/318
Host Rabbit
Reactivity Human, Mouse, Rat
Applications ELISA, IHC-P, WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:20000). Not yet tested in other applications.
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
Alternative DUSP1; CL100; MKP1; PTPN10; VH1; Dual specificity protein phosphatase 1; Dual specificity protein phosphatase hVH1; Mitogen-activated protein kinase phosphatase 1; MAP kinase phosphatase 1; MKP-1; Protein-tyrosine phosphatase CL100; DUSP4;

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Storage buffer PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Dual specificity protein phosphatase 4 encoded by DUSP14 is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which are associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. This gene product inactivates ERK1, ERK2 and JNK, is expressed in a variety of tissues, and is localized in the nucleus. Two alternatively spliced transcript variants, encoding distinct isoforms, have been observed for this gene. In addition, multiple polyadenylation sites have been reported.
Gene ID 1843/1846
Alternative DUSP1; CL100; MKP1; PTPN10; VH1; Dual specificity protein phosphatase 1; Dual specificity protein phosphatase hVH1; Mitogen-activated protein kinase phosphatase 1; MAP kinase phosphatase 1; MKP-1; Protein-tyrosine phosphatase CL100; DUSP4;
Others MKP-1/2 Polyclonal Antibody detects endogenous levels of MKP-1/2 protein.
Accession P28562/Q13115
Observed Band(KD) 41

Image & description

Fig.1. Western Blot analysis of various cells using MKP-1/2 Polyclonal Antibody diluted at 1:1000.

Fig.1. Western Blot analysis of various cells using MKP-1/2 Polyclonal Antibody diluted at 1:1000.

Fig.2. Western Blot analysis of Jurkat cells using MKP-1/2 Polyclonal Antibody diluted at 1:1000.

Fig.2. Western Blot analysis of Jurkat cells using MKP-1/2 Polyclonal Antibody diluted at 1:1000.

Fig.3. Western blot analysis of 293T lysate using MKP-1/2 antibody. Antibody was diluted at 1:1000.

Fig.3. Western blot analysis of 293T lysate using MKP-1/2 antibody. Antibody was diluted at 1:1000.

Fig.4. Western blot analysis of hela, KB, 293T, SH-SY5Y lysates using MKP-1/2 antibody. Antibody was diluted at 1:1000.

Fig.4. Western blot analysis of hela, KB, 293T, SH-SY5Y lysates using MKP-1/2 antibody. Antibody was diluted at 1:1000.

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