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Melanopsin Polyclonal Antibody

Melanopsin Polyclonal Antibody

Views(294) Publications(0) Catalog no(ABP51778)
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Specification

Product name Melanopsin Polyclonal Antibody
Immunogen Synthesized peptide derived from the C-terminal region of human Melanopsin at AA range: 400-480
Host Rabbit
Reactivity Human
Applications ELISA, IF, WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IF (1:200-1:1000), ELISA (1:5000). Not yet tested in other applications.
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
Alternative OPN4; MOP; Melanopsin; Opsin-4

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Storage buffer PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Opsins are members of the guanine nucleotide-binding protein (G protein)-coupled receptor superfamily. OPN4 encodes a photoreceptive opsin protein melanopsin that is expressed within the ganglion and amacrine cell layers of the retina. In mouse, retinal ganglion cell axons expressing this gene projected to the suprachiasmatic nucleus and other brain nuclei involved in circadian photoentrainment. In mouse, this protein is coupled to a transient receptor potential (TRP) ion channel through a G protein signaling pathway and produces a physiologic light response via membrane depolarization and increased intracellular calcium. Melanopsin functions as a sensory photopigment and may also have photoisomerase activity. Experiments with knockout mice indicate that this gene attenuates, but does not abolish, photoentrainment. Alternative splicing results in multiple transcript variants encoding different isoforms.
Gene ID 94233
Alternative OPN4; MOP; Melanopsin; Opsin-4
Others Melanopsin Polyclonal Antibody detects endogenous levels of Melanopsin protein.
Accession Q9UHM6
Observed Band(KD) 55

Image & description

Fig.1. Western Blot analysis of various cells using Melanopsin Polyclonal Antibody.

Fig.1. Western Blot analysis of various cells using Melanopsin Polyclonal Antibody.

Fig.2. Western Blot analysis of COLO205 cells using Melanopsin Polyclonal Antibody.

Fig.2. Western Blot analysis of COLO205 cells using Melanopsin Polyclonal Antibody.

Fig.3. Western blot analysis of various lysates using Melanopsin Polyclonal Antibody. Secondary antibody (catalog#: A21020) was diluted at 1:20000.

Fig.3. Western blot analysis of various lysates using Melanopsin Polyclonal Antibody. Secondary antibody (catalog#: A21020) was diluted at 1:20000.

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