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Luciferase Mouse Monoclonal Antibody (9D6)

Luciferase Mouse Monoclonal Antibody (9D6)

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Specification

Product name Luciferase Mouse Monoclonal Antibody (9D6)
Immunogen Synthetic Peptide of Luciferase
Host Mouse
Reactivity Firefly
Applications WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:1000-1:2000).
Clonality Monoclonal
Preparation method The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Storage buffer PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Luciferase from the firefly has become one of the more widely used reporter proteins for the study of gene expression. Luciferase catalyzes a bioluminescent reaction which requires the substrate luciferin as well as Mg2+ and ATP. Mixing these reagents with the cell extract containing luciferase, results in a flash of light that decays rapidly. This light can be detected by a luminometer. The total light emission is proportional to the luciferase activity of the sample.
Gene ID N/A
Others Luciferase protein detects endogenous levels of Luciferase.
Accession P08659
Observed Band(KD) 60

Image & description

Fig. Western blot analysis of Firefly Luciferase Transfected Hela Cell Lysate using Luciferase Mouse mAb diluted at 1:2000.

Fig. Western blot analysis of Firefly Luciferase Transfected Hela Cell Lysate using Luciferase Mouse mAb diluted at 1:2000.

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