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LIMK-2 Polyclonal Antibody

LIMK-2 Polyclonal Antibody

Views(15) Publications(0) Catalog no(ABP51714)
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Specification

Product name LIMK-2 Polyclonal Antibody
Immunogen Synthesized peptide derived from the Internal region of human LIMK-2 at AA range: 430-510
Host Rabbit
Reactivity Human, Mouse, Rat
Applications ELISA, IF, IHC-P, WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), IF (1:200-1:1000), ELISA (1:40000). Not yet tested in other applications.
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
Alternative LIMK2; LIM domain kinase 2; LIMK-2

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Storage buffer PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. LIM domain kinase 2 by LIMK2 is phosphorylated and activated by ROCK, a downstream effector of Rho, and the encoded protein, in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. It is thought that this pathway contributes to Rho-induced reorganization of the actin cytoskeleton. At least three transcript variants encoding different isoforms have been found for this gene.
Gene ID 3985
Alternative LIMK2; LIM domain kinase 2; LIMK-2
Others LIMK-2 Polyclonal Antibody detects endogenous levels of LIMK-2 protein.
Accession P53671
Observed Band(KD) 70

Image & description

Fig.1. Western Blot analysis of various cells using LIMK-2 Polyclonal Antibody.

Fig.1. Western Blot analysis of various cells using LIMK-2 Polyclonal Antibody.

Fig.2. Western Blot analysis of HUVEC cells using LIMK-2 Polyclonal Antibody.

Fig.2. Western Blot analysis of HUVEC cells using LIMK-2 Polyclonal Antibody.

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