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IQGAP1 Polyclonal Antibody

IQGAP1 Polyclonal Antibody

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Specification

Product name IQGAP1 Polyclonal Antibody
Immunogen Synthesized peptide derived from the N-terminal region of human IQGAP1 at AA range: 220-300
Host Rabbit
Reactivity Human, Mouse
Applications ELISA, WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), ELISA (1:20000). Not yet tested in other applications.
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
Alternative IQGAP1; KIAA0051; Ras GTPase-activating-like protein IQGAP1; p195

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Storage buffer PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background IQGAP1 encodes a member of the IQGAP family. Ras GTPase-activating-like protein IQGAP1 contains four IQ domains, one calponin homology domain, one Ras-GAP domain and one WW domain. It interacts with components of the cytoskeleton, with cell adhesion molecules, and with several signaling molecules to regulate cell morphology and motility. Expression of the protein is upregulated by gene amplification in two gastric cancer cell lines.
Gene ID 8826
Alternative IQGAP1; KIAA0051; Ras GTPase-activating-like protein IQGAP1; p195
Others IQGAP1 Polyclonal Antibody detects endogenous levels of IQGAP1 protein.
Accession P46940
Observed Band(KD) 190

Image & description

Fig.1. Western Blot analysis of various cells using IQGAP1 Polyclonal Antibody diluted at 1:1000.

Fig.1. Western Blot analysis of various cells using IQGAP1 Polyclonal Antibody diluted at 1:1000.

Fig.2. Western Blot analysis of hela cells using IQGAP1 Polyclonal Antibody diluted at 1:1000.

Fig.2. Western Blot analysis of hela cells using IQGAP1 Polyclonal Antibody diluted at 1:1000.

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