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IKKγ Polyclonal Antibody

IKKγ Polyclonal Antibody

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Specification

Product name IKKγ Polyclonal Antibody
Immunogen Synthesized peptide derived from human IKKγ around the non-phosphorylation site of S85
Host Rabbit
Reactivity Human
Applications ELISA, IF, IHC-P, WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), IF (1:200-1:1000), ELISA (1:20000). Not yet tested in other applications.
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
Alternative IKBKG; FIP3; NEMO; NF-kappa-B essential modulator; NEMO; FIP-3; IkB kinase-associated protein 1; IKKAP1; Inhibitor of nuclear factor kappa-B kinase subunit gamma; I-kappa-B kinase subunit gamma; IKK-gamma; IKKG; IkB kinase subunit gamma; NF

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Storage buffer PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background IKBKG encodes the regulatory subunit of the inhibitor of kappaB kinase (IKK) complex, which activates NF-kappaB resulting in activation of genes involved in inflammation, immunity, cell survival, and other pathways. Mutations in this gene result in incontinentia pigmenti, hypohidrotic ectodermal dysplasia, and several other types of immunodeficiencies. A pseudogene highly similar to this locus is located in an adjacent region of the X chromosome.
Gene ID 8517
Alternative IKBKG; FIP3; NEMO; NF-kappa-B essential modulator; NEMO; FIP-3; IkB kinase-associated protein 1; IKKAP1; Inhibitor of nuclear factor kappa-B kinase subunit gamma; I-kappa-B kinase subunit gamma; IKK-gamma; IKKG; IkB kinase subunit gamma; NF
Others IKKγ Polyclonal Antibody detects endogenous levels of IKKγ protein.
Accession Q9Y6K9
Observed Band(KD) 43

Image & description

Fig.1. Western Blot analysis of various cells using IKKγ Polyclonal Antibody diluted at 1:1000.

Fig.1. Western Blot analysis of various cells using IKKγ Polyclonal Antibody diluted at 1:1000.

Fig.2. Western Blot analysis of RAW264.7 cells using IKKγ Polyclonal Antibody diluted at 1:1000.

Fig.2. Western Blot analysis of RAW264.7 cells using IKKγ Polyclonal Antibody diluted at 1:1000.

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