|Product name||IGFN1 Polyclonal Antibody|
|Reactivity||Human, Mouse, Rat|
|Applications notes||Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:2000-1:5000), IHC-P (1:200-1:500).|
|Preparation method||The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen|
|Storage buffer||PBS, pH 7.4, containing 0.02% Sodium Azide as preservative and 50% Glycerol.|
|Storage instructions||Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.|
|Shipping||Gel pack with blue ice.|
|Precautions||The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.|
|Background||IGFN1 (immunoglobulin-like and fibronectin type III domain containing 1) is an 868 amino acid protein that is encoded by a gene located on chromosome 1. Chromosome 1 is the largest human chromosome spanning about 260 million base pairs and making up 8% of the human genome. There are about 3000 genes on chromosome 1, many of which are associated with a large number of diseases. Notably, the rare aging disease Hutchinson-Gilford progeria is associated with the LMNA gene which encodes lamin A. When defective, the LMNA gene product can build up in the nucleus and cause characteristic nuclear blebs. The mechanism of rapidly enhanced aging is unclear and is a topic of continuing exploration. The MUTYH gene is located on chromosome 1 and is partially responsible for familial adenomatous polyposis. Stickler syndrome, Parkinsons, Gaucher disease and Usher syndrome are also associated with chromosome 1. A breakpoint has been identified in 1q which disrupts the DISC1 gene and is linked to schizophrenia. Aberrations in chromosome 1 are found in a variety of cancers including head and neck cancer, malignant melanoma and multiple myeloma.|
|Others||The antibody detects endogenous IGFN1 protein.|
Fig.1. Western blot analysis of 1) Pig Skeletal MuscleTissue, 2) Rat Skeletal Muscle Tissue, 3) Rat Skeletal Muscle Tissue using IGFN1 Polyclonal Antibody. Secondary antibody (catalog#: A21020) was diluted at 1:20000.
Fig.2. Immunohistochemical analysis of paraffin-embedded Mouse Skeletal MuscleTissue using IGFN1 Polyclonal Antibody.