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Human VEGF165 protein

Human VEGF165 protein

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Product name Human VEGF165 protein
Immunogen PRP100239
Sequence Amino acid sequence derived from human VEGF 165 isoform (P15692-4) (Met1-Arg191) was expressed. Human and Cynomolgus VEGF165 sequences are identical.
Activity Measured in a cell proliferation assay using human umbilical vein endothelial cells (HUVEC). The ED50 for this effect is typically 3-12 ng/ml. The ED50 for this effect is typically 3-12 ng/ml .
Protein length The recombinant human VEGF consists of 165 amino acids after removal of the signal peptide and predicts a molecular mass of 19.2 kDa. Due to different glycosylation, the apparent molecular mass of rhVEGF165 is approximately 20 and 22 kDa in SDS-PAGE under reducing conditions, corresponding to the monomer.
Preparation method Baculovirus-Insect Cells
Purity > 97 % as determined by SDS-PAGE
Alternative MVCD1; VEGF; VEGF165; VPF; Vascular endothelial growth factor A; VEGFA

Product Properties

Formulation Lyophilized from sterile PBS, pH 7.4
Molecular weight 19.2 kDa
Usage notes Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Human VEGF165 protein in sterile ddH2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Storage instructions Lyophilized Human VEGF165 proteinshould be stored desiccated below -20°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.61% HSA or BSA). Please prevent freeze-thaw cycles.
Shipping The product is shipped at ambient temperature.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Vascular endothelial growth factor (VEGF), also known as vascular permeability factor (VPF) and VEGF-A, is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult. It is a member of the platelet-derived growth factor (PDGF)/vascular endothelial growth factor (VEGF) family and often exists as a disulfide-linked homodimer. VEGF-A protein is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, inhibiting apoptosis and tumor growth. VEGF-A protein is also a vasodilator that increases microvascular permeability, thus it was originally referred to as vascular permeability factor.
Gene ID 7422
Alternative MVCD1; VEGF; VEGF165; VPF; Vascular endothelial growth factor A; VEGFA
Others The Endotoxin level is less than 1.0 EU per μg of the protein as determined by the LAL method
Accession P15692-4

Image & description

Fig.SDS-PAGE analysis of Human VEGF165 protein.

Fig.SDS-PAGE analysis of Human VEGF165 protein.

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