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Human VEGF121 protein

Human VEGF121 protein

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Specification

Product name Human VEGF121 protein
Sequence Amino acid sequence derived from human VEGF 121 isoform (P15692-9) (Met1-Arg147) was expressed.
Activity Measured by its binding ability in a functional ELISA. Immobilized human VEGF121 at 10μg/mL (100μL/well) can bind human VEGFR2-Fc, the EC50 of human VEGFR2-Fc is 5-50 ng/mL.
Protein length The recombinant human VEGF consists of 121 amino acids and has a predicted molecular mass of 14 kDa. The apparent molecular mass of the human VEGF 121 is approximately 19.9 and 17.0 kDa in SDS-PAGE under reducing conditions due to glycosylation.
Preparation method HEK293 Cells
Purity (55.6 + 41.6) % as determined by SDS-PAGE
Alternative MVCD1; VEGF; VEGF165; VPF; Vascular endothelial growth factor A; VEGFA

Product Properties

Formulation Lyophilized from sterile PBS, pH 7.4.
Molecular weight 14 kDa
Usage notes Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Human VEGF121 protein in sterile ddH2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Storage instructions Lyophilized Human VEGF121 protein product should be stored desiccated below -20°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Shipping The product is shipped at ambient temperature.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Vascular endothelial growth factor (VEGF), also known as vascular permeability factor (VPF) and VEGF-A, is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult. It is a member of the platelet-derived growth factor (PDGF)/vascular endothelial growth factor (VEGF) family and often exists as a disulfide-linked homodimer. VEGF-A protein is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, inhibiting apoptosis and tumor growth. VEGF-A protein is also a vasodilator that increases microvascular permeability, thus it was originally referred to as vascular permeability factor.
Gene ID 7422
Alternative MVCD1; VEGF; VEGF165; VPF; Vascular endothelial growth factor A; VEGFA
Others The Endotoxin level is less than 1.0 EU per μg of the protein as determined by the LAL method
Accession P15692-9

Image & description

Fig.SDS-PAGE analysis of Human VEGF121 protein.

Fig.SDS-PAGE analysis of Human VEGF121 protein.

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