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Human VEGF-C protein, His Tag

Human VEGF-C protein, His Tag

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Specification

Product name Human VEGF-C protein, His Tag
Sequence Amino acid sequence derived from mature form of human VEGFC (NP_005420.1) corresponding to amino acid (Thr 103-Arg 227) was expressed with a C-terminal polyhistidine tag.
Activity 1. Measured by its binding ability in a functional ELISA . Scatchard analysis showed the affinity constant (Kd) of recombinant human VEGF-C bound to recombinant human VEGFR3 was 1.4 nM . 2. Measured in a cell proliferation assay using human umbilical vein endothelial cells (HUVEC). The ED50 for this effect is 0.1-0.5μg/mL.
Protein length The recombinant mature form of human VEGFC consists of 136 amino acids and has a predicted molecular mass of 15.5 kDa. In SDS-PAGE under reducing conditions, it migrates with an apparent molecular mass of 22-24 kDa due to glycosylation.
Preparation method HEK293 Cells
Purity > 97 % as determined by SDS-PAGE
Alternative Flt4-L; LMPH1D; VEGF-C; VRP; Vascular endothelial growth factor C; VEGFC

Product Properties

Formulation Lyophilized from sterile PBS, pH 7.4
Molecular weight 15.5 kDa
Usage notes Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Human VEGF-C protein, His Tag in sterile ddH2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Storage instructions Lyophilized Human VEGF-C protein, His Tag product should be stored desiccated below -20°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Shipping The product is shipped at ambient temperature.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Vascular endothelial growth factor C (VEGF-C) is a member of the VEGF family. Upon biosynthesis, VEGF-C protein is secreted as a non-covalent momodimer in an anti-parellel fashion. VEGF-C protein is a dimeric glycoprotein, as a ligand for two receptors, VEGFR-3 (Flt4), and VEGFR-2. VEGF-C may function in angiogenesis of the venous and lymphatic vascular systems during embryogenesis. VEGF-C protein is over-expressed in various human cancers including breast cancer and prostate cancer. VEGF-C/VEGFR-3 axis, through different signaling pathways, plays a critical role in cancer progression by regulating different cellular functions, such as invasion, proliferation, and resistance to chemotherapy. Thus, targeting the VEGF-C/VEGFR-3 axis may be therapeutically significant for certain types of tumors.
Gene ID 7424
Alternative Flt4-L; LMPH1D; VEGF-C; VRP; Vascular endothelial growth factor C; VEGFC
Others The Endotoxin level is less than 1.0 EU per μg of the protein as determined by the LAL method
Accession NP_005420.1

Image & description

Fig.SDS-PAGE analysis of Human VEGF-C protein, His Tag.

Fig.SDS-PAGE analysis of Human VEGF-C protein, His Tag.

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