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Human Tumor necrosis factor α IgG antibody (TNFA-Ab-IgG) ELISA Kit

Human Tumor necrosis factor α IgG antibody (TNFA-Ab-IgG) ELISA Kit

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Specification

Product name Human Tumor necrosis factor α IgG antibody (TNFA-Ab-IgG) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Tumor necrosis factor α IgG antibody (TNFA-Ab-IgG) ELISA Kit employs a two-site sandwich ELISA to quantitate TNFA-Ab-IgG in samples. An antibody specific for TNFA-Ab-IgG has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTNFA-Ab-IgG present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNFA-Ab-IgG is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNFA-Ab-IgG bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative TNFA-Ab-IgG; antibody for DADB-70P7.1; DIF; TNF-alpha; TNFA; TNFSF2; APC1 protein; TNF superfamily; member 2; TNF; macrophage-derived; TNF; monocyte-derived; cachectin; tumor necrosis factor alpha

Product Properties

Kit components • Human Tumor necrosis factor α IgG antibody microplate
• Human Tumor necrosis factor α IgG antibody standard
• Human Tumor necrosis factor α IgG antibody detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Tumor necrosis factor α IgG antibody (TNFA-Ab-IgG) ELISA Kit has high sensitivity and excellent specificity for detection of Human TNFA-Ab-IgG. No significant cross-reactivity or interference between Human TNFA-Ab-IgG and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background TNFa  is synthesized as a 26 kDa, type II transmembrane protein that is 233 amino acids in length. It contains a 30 amino acid (aa) cytoplasmic domain, a 26 aa transmembrane segment, and a 177 aa extracellular region. TNFa is assembled intracellularly to form a transmembrane, non-covalently-linked homotrimeric protein. The 157 aa residue soluble form of TNFa (sTNF-αis released from the C-terminus of the transmembrane protein through the activity of TNFa-converting enzyme (TACE), a membrane -bound disintegrin metalloproteinase. Rat cells known to express TNF-αinclude B cells, colonic columnar epithelial cells, NK and CD3 CD56 hepatic natural T cells, macrophages, monocytes and monocyte-derived dendritic cells, CD4 and CD8 T cells, mast cells, neutrophils, keratinocytes, plasma cells, and adipocytes.
Alternative TNFA-Ab-IgG; antibody for DADB-70P7.1; DIF; TNF-alpha; TNFA; TNFSF2; APC1 protein; TNF superfamily; member 2; TNF; macrophage-derived; TNF; monocyte-derived; cachectin; tumor necrosis factor alpha

Image & description

Fig.1. Human Tumor necrosis factor α IgG antibody (TNFA-Ab-IgG) Standard Curve.

Fig.1. Human Tumor necrosis factor α IgG antibody (TNFA-Ab-IgG) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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