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Human Trypsinogen-2 (TRY2) ELISA Kit

Human Trypsinogen-2 (TRY2) ELISA Kit

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Specification

Product name Human Trypsinogen-2 (TRY2) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Trypsinogen-2 (TRY2) ELISA Kit employs a two-site sandwich ELISA to quantitate TRY2 in samples. An antibody specific for TRY2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTRY2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TRY2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TRY2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative TRY2

Product Properties

Kit components • Human Trypsinogen-2 microplate
• Human Trypsinogen-2 standard
• Human Trypsinogen-2 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Trypsinogen-2 (TRY2) ELISA Kit has high sensitivity and excellent specificity for detection of Human TRY2. No significant cross-reactivity or interference between Human TRY2 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background In the ileum, PRSS2 may be involved in defensin processing, including DEFA5.PRSS2 is a member of the trypsin family of serine proteases. This enzyme is secreted by the pancreas and cleaved to its active form in the small intestine. It is active on peptide linkages involving the carboxyl group of lysine or arginine. This gene and several other trypsinogen genes are localized to the T cell receptor beta locus on chromosome 7.Trypsin is a serine protease found in the digestive system of many vertebrates, where it hydrolyses proteins.Rowen et al. (1996) mapped the gene corresponding to the third pancreatic trypsinogen cDNA by fluorescence in situ hybridization. They used a cosmid clone containing 3 trypsinogen genes.Strong hybridization to chromosome 7 and weaker hybridization to chromosome 9 were observed. They isolated and partially sequenced 4 cosmid clones from the chromosome 9 region. They found that the region represents a duplication and translocation of a DNA segment from the 3-prime end of the TCRB locus that includes at least 7 V(beta) elements and a functional trypsinogen gene denoted T9.
Alternative TRY2

Image & description

Fig.1. Human Trypsinogen-2 (TRY2) Standard Curve.

Fig.1. Human Trypsinogen-2 (TRY2) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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