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Human Transfusion transimitted virus antibody (TTV-Ab) ELISA Kit

Human Transfusion transimitted virus antibody (TTV-Ab) ELISA Kit

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Specification

Product name Human Transfusion transimitted virus antibody (TTV-Ab) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Transfusion transimitted virus antibody (TTV-Ab) ELISA Kit employs the competitive enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to TTV-Ab. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated TTV-Ab and incubated. The competitive inhibition reaction is launched between with HRP labeled TTV-Ab and unlabeled TTV-Ab with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of TTV-Ab in the sample. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative TTV-Ab; hepatitis H virus

Product Properties

Kit components • Human Transfusion transimitted virus antibody microplate
• Human Transfusion transimitted virus antibody standard
• Human Transfusion transimitted virus antibody detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Transfusion transimitted virus antibody (TTV-Ab) ELISA Kit has high sensitivity and excellent specificity for detection of Human TTV-Ab. No significant cross-reactivity or interference between Human TTV-Ab and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Exostosin-1 is an endoplasmic reticulum-resident type II transmembrane glycosyltransferase involved in the chain elongation step of heparan sulfate biosynthesis. Mutations in this gene cause the type I form of multiple exostoses.By screening a human chondrocyte cDNA library with cosmids that spanned breakpoints on chromosome 8q identified in patients with multiple exostoses type I, Ahn et al. (1995) identified a cDNA encoding a putative 746-amino acid protein with a molecular mass of 86.3 kD. Northern blot analysis detected expression of a 3.4-kb transcript in all tissues tested, with highest levels in liver. The authors noted that the breakpoint region in the EXT1 gene contains 2 identical polypyrimidine tracts (CCCCCCT) that are known to be deletion hotspots, similar to the retinoblastoma gene.
Alternative TTV-Ab; hepatitis H virus

Image & description

Fig.1. Human Transfusion transimitted virus antibody (TTV-Ab) Standard Curve.

Fig.1. Human Transfusion transimitted virus antibody (TTV-Ab) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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