|Product name||Human Total protein S (TPS) ELISA Kit|
|Applications notes||This Human Total protein S (TPS) ELISA Kit employs a two-site sandwich ELISA to quantitate TPS in samples. An antibody specific for TPS has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTPS present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TPS is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TPS bound in the initial step. The color development is stopped and the intensity of the color is measured.|
|SampleType||Cell culture supernatants, Other biological fluids, Plasma, Serum|
|Assay type||Sandwich ELISA (quantitative)|
|Assay duration||Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.|
|Kit components||• Human Total protein S microplate
• Human Total protein S standard
• Human Total protein S detect antibody
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
|Features & Benefits||Human Total protein S (TPS) ELISA Kit has high sensitivity and excellent specificity for detection of Human TPS. No significant cross-reactivity or interference between Human TPS and analogues was observed.|
|Calibration range||Please inquire|
|Limit of detection||Please inquire|
|Usage notes||• Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
|Storage instructions||The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.|
|Shipping||Gel pack with blue ice.|
|Precautions||The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.|
|Background||TPS is the specific M3 epitope of TPA has been used as a prognostic parameter for some neoplasms since it was isolated in the serum or urine of some cancer patients.TPS is most frequently elevated in malignant tissues. Serum TPS levels not only reflect tumor mass but also tumor activity and are related with proliferation more than necrosis. Furthermore, elevated TPS levels can also be detected in some benign events such as liver failure, renal failure, gestati-on, generalized infection, and diabetes mellitus(DM). TPS levels in cystic fluid, peritoneal fluid, and blood could be used as a marker for the management of ovarian mass.The value of serum TPS as a complement to CA 19-9 in the detection of pancreatic carcinoma was determined prospectively. TPS and CA 19-9 levels obtained at the time of diagnosis in patients suspected of having chronic pancreatitis or pancreatic carcinoma were evaluated in receiver operating characteristic (ROC) curve analysis.|
Fig.1. Human Total protein S (TPS) Standard Curve.
Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.