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Human TNF-beta protein

Human TNF-beta protein

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Specification

Product name Human TNF-beta protein
Sequence Amino acid sequence derived from mature form of human TNFβ (P01374) (Leu 35-Leu 205) was expressed and purified, with an initial Met at the N-terminus.
Activity Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is typically 0.01-0.1 ng/ml.
Protein length The recombinant human TNFβ comprises 172 amino acids and predicts a molecular mass of 18.8 kDa as estimated in SDS-PAGE under reducing conditions.
Preparation method E. coli
Purity > 97 % as determined by SDS-PAGE
Alternative LT; TNFB; TNFSF1; Lymphotoxin-alpha; LTA

Product Properties

Formulation Lyophilized from sterile 50mM Tris, pH 8.0
Molecular weight 18.8 kDa
Usage notes Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Human TNF-beta protein in sterile ddH2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Storage instructions Lyophilized Human TNF-beta protein product should be stored desiccated below -20°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Shipping The product is shipped at ambient temperature.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Lymphotoxin-alpha, also known as LT-alpha, TNF-beta, Tumor necrosis factor ligand superfamily member 1, LTA TNFSF1 and TNFB, is a secreted protein which belongs to the tumor necrosis factor family. TNF-beta/TNFSF1/Lymphotoxin alpha is a highly inducible, secreted, and exists as homotrimeric molecule. It is a cytokine that in its homotrimeric form binds to TNFRSF1A / TNFR1, TNFRSF1B / TNFBR and TNFRSF14 / HVEM. In its heterotrimeric form with LTB, TNF-beta/TNFSF1/Lymphotoxin alpha binds to TNFRSF3 / LTBR. Lymphotoxin is produced by lymphocytes and cytotoxic for a wide range of tumor cells. TNF-beta/TNFSF1/Lymphotoxin alpha forms heterotrimers with lymphotoxin-beta which anchors lymphotoxin-alpha to the cell surface. It mediates a large variety of inflammatory, immunostimulatory, and antiviral responses. TNF-beta/TNFSF1/Lymphotoxin alpha is also involved in the formation of secondary lymphoid organs during development and plays a role in apoptosis. Genetic variations in TNF-beta/TNFSF1/Lymphotoxin alpha are a cause of susceptibility psoriatic arthritis which is an inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy.
Gene ID 4049
Alternative LT; TNFB; TNFSF1; Lymphotoxin-alpha; LTA
Accession P01374

Image & description

Fig.SDS-PAGE analysis of Human TNF-beta protein.

Fig.SDS-PAGE analysis of Human TNF-beta protein.

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