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Human Thyroid transcription factor 1 (TITF1) ELISA Kit

Human Thyroid transcription factor 1 (TITF1) ELISA Kit

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Specification

Product name Human Thyroid transcription factor 1 (TITF1) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Thyroid transcription factor 1 (TITF1) ELISA Kit employs a two-site sandwich ELISA to quantitate TITF1 in samples. An antibody specific for TITF1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTITF1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TITF1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TITF1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative TITF1; BCH; BHC; NK-2; NKX2.1; NKX2A; TEBP; TITF1; TTF-1; TTF1; NK-2 homolog A; thyroid nuclear factor; thyroid transcription factor 1

Product Properties

Kit components • Human Thyroid transcription factor 1 microplate
• Human Thyroid transcription factor 1 standard
• Human Thyroid transcription factor 1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Thyroid transcription factor 1 (TITF1) ELISA Kit has high sensitivity and excellent specificity for detection of Human TITF1. No significant cross-reactivity or interference between Human TITF1 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Thyroid transcription factor-1 (TTF-1) is a protein that regulates transcription of genes specific for the thyroid, lung, and diencephalon. It is also known as thyroid specific enhancer binding protein. It is used in anatomic pathology as a marker to determine if a tumor arises from the lung or thyroid.TTF-1 positive cells are found in the lung as type II pneumocytes and Clara cells. In the thyroid, follicular and parafollicular cells are also positive for TTF-1.For lung cancers, adenocarcinomas are usually positive, while squamous cell carcinomas and large cell carcinomas are rarely positive. Small cell carcinomas (of any primary site) are usually positive. TTF1 is more than merely a clinical marker of lung adenocarcinoma. It plays an active role in sustaining lung cancer cells in view of the experimental observation that it is mutated in lung cancer.
Gene ID 403940
Alternative TITF1; BCH; BHC; NK-2; NKX2.1; NKX2A; TEBP; TITF1; TTF-1; TTF1; NK-2 homolog A; thyroid nuclear factor; thyroid transcription factor 1
Accession P43698

Image & description

Fig.1. Human Thyroid transcription factor 1 (TITF1) Standard Curve.

Fig.1. Human Thyroid transcription factor 1 (TITF1) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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