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Human Thrombopoietin protein, His Tag

Human Thrombopoietin protein, His Tag

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Specification

Product name Human Thrombopoietin protein, His Tag
Sequence Amino acid sequence derived from mature form of human THPO (NP_000451.1) (Ser 22-Gly 353) was fused with a polyhistidine tag at the N-terminus.
Activity Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. The ED50 for this effect is typically 10-60 ng/ml.
Protein length The recombinant human THPO consists of 348 amino acids with the predicted molecular mass of 37.6 kDa. As a result of glycosylation, rhTHPO migrates as an approximately 80 kDa band in SDS-PAGE under reducing conditions.
Preparation method HEK293 Cells
Purity > 95 % as determined by SDS-PAGE
Alternative MGDF; MKCSF; ML; MPLLG; THCYT1; TPO; Thrombopoietin; THPO

Product Properties

Formulation Lyophilized from sterile PBS, pH 7.4
Molecular weight 37.6 kDa
Usage notes Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Human Thrombopoietin protein, His Tag in sterile ddH2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Storage instructions Lyophilized Human Thrombopoietin protein, His Tag product should be stored desiccated below -20°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Shipping The product is shipped at ambient temperature.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Thrombopoietin (TPO or THPO), also known as myeloproliferative leukemia virus ligand (c-Mpl), is a hematopoietic growth factor belonging to the EPO/TPO family. The thrombopoietin protein is produced mainly by the liver and the kidney that regulates the production of platelets by the bone marrow. Thrombopoietin protein stimulates both proliferation of progenitor megakaryocytes and their maturation to platelet-producing megakaryocytes, and also accelerates the recovery of platelets. Thrombopoietin protein is involved in cardiovascular disease as it regulates megakaryocyte development and enhances platelet adhesion/aggregation. It has been identified that surface c-MPL, the receptor for thrombopoietin protein, binds to the ligand and mediates the action.
Gene ID 7066
Alternative MGDF; MKCSF; ML; MPLLG; THCYT1; TPO; Thrombopoietin; THPO
Others The Endotoxin level is less than 1.0 EU per μg of the protein as determined by the LAL method
Accession NP_000451.1

Image & description

Fig.SDS-PAGE analysis of Human Thrombopoietin protein, His Tag.

Fig.SDS-PAGE analysis of Human Thrombopoietin protein, His Tag.

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