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Human T-lymphocyte surface antigen Ly-9 (LY9) ELISA Kit

Human T-lymphocyte surface antigen Ly-9 (LY9) ELISA Kit

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Specification

Product name Human T-lymphocyte surface antigen Ly-9 (LY9) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human T-lymphocyte surface antigen Ly-9 (LY9) ELISA Kit employs a two-site sandwich ELISA to quantitate LY9 in samples. An antibody specific for LY9 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLY9 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LY9 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LY9 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative LY9; RP11-312J18.1; CD229; SLAMF3; hly9; mLY9; T-lymphocyte surface antigen Ly-9; cell-surface molecule Ly-9

Product Properties

Kit components • Human T-lymphocyte surface antigen Ly-9 microplate
• Human T-lymphocyte surface antigen Ly-9 standard
• Human T-lymphocyte surface antigen Ly-9 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human T-lymphocyte surface antigen Ly-9 (LY9) ELISA Kit has high sensitivity and excellent specificity for detection of Human LY9. No significant cross-reactivity or interference between Human LY9 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Lymphocyte antigen 9, also known as LY9, has also recently been designated CD229 . LY9 has been shown to interact with SH2D1A.By sequence analysis of cDNA clones, Sandrin et al. (1992) demonstrated that the mouse lymphocyte surface antigen Ly9 is a novel member of a subgroup of the immunoglobulin superfamily, together with the antigens CD2, BCM1 (CD48), and LFA3 (CD58). Kingsmore et al. (1995) noted that, though the function of Ly9 was unknown, other subgroup members are involved in adhesion reactions between T lymphocytes and accessory cells. CD2, CD48, and CD58 are also distinguished by homophilic interactions with one another; thus, human CD48 and CD58 are both ligands for CD2.
Gene ID 4063
Alternative LY9; RP11-312J18.1; CD229; SLAMF3; hly9; mLY9; T-lymphocyte surface antigen Ly-9; cell-surface molecule Ly-9
Accession Q9HBG7

Image & description

Fig.1. Human T-lymphocyte surface antigen Ly-9 (LY9) Standard Curve.

Fig.1. Human T-lymphocyte surface antigen Ly-9 (LY9) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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