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Human Serine beta-lactamase-like protein LACTB, mitochondrial (LACTB) ELISA Kit

Human Serine beta-lactamase-like protein LACTB, mitochondrial (LACTB) ELISA Kit

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Specification

Product name Human Serine beta-lactamase-like protein LACTB, mitochondrial (LACTB) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Serine beta-lactamase-like protein LACTB, mitochondrial (LACTB) ELISA Kit employs a two-site sandwich ELISA to quantitate LACTB in samples. An antibody specific for LACTB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLACTB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LACTB is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LACTB bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative LACTB; FLJ14902; G24; MRPL56; mitochondrial 39S ribosomal protein L56; mitochondrial ribosomal protein L56; serine beta lactamase-like protein LACTB

Product Properties

Kit components • Human Serine beta-lactamase-like protein LACTB, mitochondrial microplate
• Human Serine beta-lactamase-like protein LACTB, mitochondrial standard
• Human Serine beta-lactamase-like protein LACTB, mitochondrial detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Serine beta-lactamase-like protein LACTB, mitochondrial (LACTB) ELISA Kit has high sensitivity and excellent specificity for detection of Human LACTB. No significant cross-reactivity or interference between Human LACTB and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background LACTB encodes a protein from the large 39S subunit of the mitochondrial ribosome. LACTb has some sequence similarity to prokaryotic beta-lactamases but most of the residues that are responsible for the beta-lactamase activity are not conserved between the two proteins.The deduced 551-amino acid Lactb protein contains an N-terminal transmembrane domain and an alpha/beta-hydrolase fold catalytic site. LACTB, which encodes a predicted 549-amino acid protein that shares 90% amino acid identity with mouse Lactb. Northern blot analysis of mouse tissues detected expression at high levels in liver, heart, and skeletal muscle, with lower levels in kidney, testis, brain, lung, and spleen. Northern blot analysis of human tissues detected a 2.3-kb transcript expressed at high levels in heart and skeletal muscle.
Gene ID 114294
Alternative LACTB; FLJ14902; G24; MRPL56; mitochondrial 39S ribosomal protein L56; mitochondrial ribosomal protein L56; serine beta lactamase-like protein LACTB
Accession P83111

Image & description

Fig.1. Human Serine beta-lactamase-like protein LACTB, mitochondrial (LACTB) Standard Curve.

Fig.1. Human Serine beta-lactamase-like protein LACTB, mitochondrial (LACTB) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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