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Human SCF (aa 1-189) protein, His Tag

Human SCF (aa 1-189) protein, His Tag

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Specification

Product name Human SCF (aa 1-189) protein, His Tag
Sequence Amino acid sequence derived from amino acid (Met 1-Ala 189) of human SCF (P21583-1) extracellular domain was expressed, with a polyhistidine tag at the C-terminus.
Activity Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is 2-8 ng/mL.
Protein length The secreted recombinant human SCF (aa 1-189) consists of 175 amino acids and predicts a molecular mass of 19.9 kDa. The apparent molecular mass of rh SCF is approximately 22 kDa in SDS-PAGE under reducing conditions.
Preparation method Baculovirus-Insect Cells
Purity > 92 % as determined by SDS-PAGE
Alternative C-kit ligand; DCUA; FPH2; FPHH; Kitl; KL-1; MGF; SCF; SF; SHEP7; Kit ligand; KITLG

Product Properties

Formulation Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 8.0
Molecular weight 19.9 kDa
Usage notes Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Human SCF (aa 1-189) protein, His Tag in sterile ddH2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Storage instructions Lyophilized Human SCF (aa 1-209) protein, His Tag product should be stored desiccated below -20°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Shipping The product is shipped at ambient temperature.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Similar to Kit ligand precursor (C-kit ligand) , also known as Stem cell factor (SCF), Mast cell growth factor (MGF) or Hematopoietic growth factor KL. SCF/C-kit ligand is the ligand of the tyrosine-kinase receptor encoded by the KIT locus. This ligand is a pleiotropic factor that acts in utero in germ cell and neural cell development, and hematopoiesis, all believed to reflect a role in cell migration. In adults, it functions pleiotropically, while mostly noted for its continued requirement in hematopoiesis. SCF/C-kit ligand stimulates the proliferation of mast cells. This protein is able to augment the proliferation of both myeloid and lymphoid hematopoietic progenitors in bone marrow culture. It may act synergistically with other cytokines, probably interleukins SCF/C-kit ligand is the ligand for the tyrosine kinase receptor c-kit, which is expressed on both primitive and mature hematopoietic progenitor cells. In vitro, SCF/C-kit ligand synergizes with other growth factors, such as granulocyte colony-stimulating factor (G-CSF), granulocyte macrophage- colony- stimulating factor, and interleukin-3 to stimulate the proliferation and differentiation of cells of the lymphoid, myeloid, erythroid, and megakaryocytic lineages. In vivo, SCF/C-kit also synergizes with other growth factors and has been shown to enhance the mobilization of peripheral blood progenitor cells in combination with G-CSF. In phase I/II clinical studies administration of the combination of SCF and G-CSF resulted in a two- to threefold increase in cells that express the CD34 antigen compared with G-CSF alone.
Gene ID 4254
Alternative C-kit ligand; DCUA; FPH2; FPHH; Kitl; KL-1; MGF; SCF; SF; SHEP7; Kit ligand; KITLG
Others The Endotoxin level is less than 1.0 EU per μg of the protein as determined by the LAL method
Accession P21583-1

Image & description

Fig.SDS-PAGE analysis of Human SCF (aa 1-189) protein, His Tag.

Fig.SDS-PAGE analysis of Human SCF (aa 1-189) protein, His Tag.

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