Login Register
English
0

Cart

$ 0

Human Salivary duct autoantibody (SDA) ELISA Kit

Human Salivary duct autoantibody (SDA) ELISA Kit

Views(114) Publications(0) Catalog no(KTE62483)
Datasheet Print Share Email Share
  • Specification
  • FAQ
  • Publications(0)
  • Comments(0)

Specification

Product name Human Salivary duct autoantibody (SDA) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Salivary duct autoantibody (SDA) ELISA Kit employs the competitive enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to SDA. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated SDA and incubated. The competitive inhibition reaction is launched between with HRP labeled SDA and unlabeled SDA with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of SDA in the sample. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative SDA

Product Properties

Kit components • Human Salivary duct autoantibody microplate
• Human Salivary duct autoantibody standard
• Human Salivary duct autoantibody detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Salivary duct autoantibody (SDA) ELISA Kit has high sensitivity and excellent specificity for detection of Human SDA. No significant cross-reactivity or interference between Human SDA and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background B4GALNT2 catalyzes the last step in the biosynthesis of the human Sd(a) antigen through the addition of an N-acetylgalactosamine residue via a beta-1,4 linkage to a subterminal galactose residue substituted with an alpha-2,3-linked sialic acid. B4GALNT2 also catalyzes the last step in the biosynthesis of the Cad antigen. The longer transcript encodes a deduced 566-amino acid protein with a calculated molecular mass of 63.3 kD. It contains an N-terminal cytosolic domain, followed by a transmembrane domain and a long catalytic domain. The shorter transcript encodes a deduced 506-amino acid protein with calculated molecular mass of 57.0 kD. It has a different N-terminal cytosolic domain than the full-length form. Northern blot analysis detected B4GALNT2 transcripts of 8.8, 6.1, 4.7, 3.8, and 1.65 kb.
Alternative SDA

Image & description

Fig.1. Human Salivary duct autoantibody (SDA) Standard Curve.

Fig.1. Human Salivary duct autoantibody (SDA) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

This product has been cited in0publications

Reviews

There are no reviews yet.

Be the first to review “Human Salivary duct autoantibody (SDA) ELISA Kit”