|Product name||Human Rota Virus (RV) ELISA kit|
|Applications notes||This Human Rota Virus (RV) ELISA kit employs a two-site sandwich ELISA to quantitate RV in samples. An antibody specific for RV has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyRV present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RV is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RV bound in the initial step. The color development is stopped and the intensity of the color is measured.|
|SampleType||Cell culture supernatants, Other biological fluids, Plasma, Serum|
|Assay type||Sandwich ELISA (quantitative)|
|Assay duration||Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.|
|Kit components||• Human Rota Virus microplate
• Human Rota Virus standard
• Human Rota Virus detect antibody
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
|Features & Benefits||Human Rota Virus (RV) ELISA kit has high sensitivity and excellent specificity for detection of Human RV. No significant cross-reactivity or interference between Human RV and analogues was observed.|
|Calibration range||Please inquire|
|Limit of detection||Please inquire|
|Usage notes||• Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
|Storage instructions||The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.|
|Shipping||Gel pack with blue ice.|
|Precautions||The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.|
|Background||Rotavirus is a virus that infects the intestinal tract. You can get rotavirus more than once, but the first infection is usually the worst. This infection causes stomach upset and diarrhea. Babies and very young children who have rotavirus infections need to be watched closely, because they can become dehydrated very quickly. Dehydration occurs when the body loses water more quickly than it is replaced. When your child becomes dehydrated, severe health problems can arise. Rotavirus infections spread easily. Outbreaks usually occur in the winter and early spring. Rotavirus infections often spread in settings where many children are together, such as day care centers.|
Fig.1. Human Rota Virus (RV) Standard Curve.
Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.