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Human Retinoic acid receptor RXR-gamma (RXRG) ELISA Kit

Human Retinoic acid receptor RXR-gamma (RXRG) ELISA Kit

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Specification

Product name Human Retinoic acid receptor RXR-gamma (RXRG) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Retinoic acid receptor RXR-gamma (RXRG) ELISA Kit employs a two-site sandwich ELISA to quantitate RXRG in samples. An antibody specific for RXRG has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyRXRG present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RXRG is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RXRG bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative RXRG; NR2B3; RXRC; retinoic acid receptor RXR-gamma; retinoid X receptor-gamma

Product Properties

Kit components • Human Retinoic acid receptor RXR-gamma microplate
• Human Retinoic acid receptor RXR-gamma standard
• Human Retinoic acid receptor RXR-gamma detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Retinoic acid receptor RXR-gamma (RXRG) ELISA Kit has high sensitivity and excellent specificity for detection of Human RXRG. No significant cross-reactivity or interference between Human RXRG and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background RXR-gamma), also known as NR2B3 (nuclear receptor subfamily 2, group B, member 3) is a nuclear receptor encoded by the RXRG gene. This gene encodes a member of the retinoid X receptor (RXR) family of nuclear receptors which are involved in mediating the antiproliferative effects of retinoic acid (RA). This receptor forms homodimers with the retinoic acid, thyroid hormone, and vitamin D receptors, increasing both DNA binding and transcriptional function on their respective response elements. This gene is expressed at significantly lower levels in non-small cell lung cancer cells. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
Gene ID 6258
Alternative RXRG; NR2B3; RXRC; retinoic acid receptor RXR-gamma; retinoid X receptor-gamma
Accession P48443

Image & description

Fig.1. Human Retinoic acid receptor RXR-gamma (RXRG) Standard Curve.

Fig.1. Human Retinoic acid receptor RXR-gamma (RXRG) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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