|Product name||Human Receptor III for the Fc region of immunoglobulin G (FcGRIII) ELISA Kit|
|Applications notes||This Human Receptor III for the Fc region of immunoglobulin G (FcGRIII) ELISA Kit employs a two-site sandwich ELISA to quantitate FcGRIII in samples. An antibody specific for FcGRIII has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyFcGRIII present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FcGRIII is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FcGRIII bound in the initial step. The color development is stopped and the intensity of the color is measured.|
|SampleType||Cell culture supernatants, Other biological fluids, Plasma, Serum|
|Assay type||Sandwich ELISA (quantitative)|
|Assay duration||Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.|
|Kit components||• Human Receptor III for the Fc region of immunoglobulin G microplate
• Human Receptor III for the Fc region of immunoglobulin G standard
• Human Receptor III for the Fc region of immunoglobulin G detect antibody
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
|Features & Benefits||Human Receptor III for the Fc region of immunoglobulin G (FcGRIII) ELISA Kit has high sensitivity and excellent specificity for detection of Human FcGRIII. No significant cross-reactivity or interference between Human FcGRIII and analogues was observed.|
|Calibration range||Please inquire|
|Limit of detection||Please inquire|
|Usage notes||• Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
|Storage instructions||The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.|
|Shipping||Gel pack with blue ice.|
|Precautions||The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.|
|Background||Aitman et al. showed that copy number variation of the orthologous rat and human Fcgr3 genes is a determinant of susceptibility to immunologically mediated glomerulonephritis. Positional cloning identified loss of the rat-specific Fcgr3 paralog 'Fcgr3-related sequence' (Fcgr3rs) as a determinant of macrophage overactivity and glomerulonephritis in Wistar Kyoto rats. In humans, low copy number of FCGR3B, an ortholog of rat Fcgr3, was associated with glomerulonephritis in the autoimmune disorder systemic lupus erythematosus (SLE. Aitman et al. concluded that their finding that gene copy number polymorphism predisposes to immunologically mediated renal disease in 2 mammalian species provides direct evidence for the importance of genome plasticity in the evolution of genetically complex phenotypes, including susceptibility to common human disease.|
Fig.1. Human Receptor III for the Fc region of immunoglobulin G (FcGRIII) Standard Curve.
Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.