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Human Pulmonary surfactant-associated protein D (SP-D) ELISA Kit

Human Pulmonary surfactant-associated protein D (SP-D) ELISA Kit

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Specification

Product name Human Pulmonary surfactant-associated protein D (SP-D) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Pulmonary surfactant-associated protein D (SP-D) ELISA Kit employs a two-site sandwich ELISA to quantitate SFTPD in samples. An antibody specific for SFTPD has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySFTPD present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SFTPD is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SFTPD bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative SFTPD; COLEC7; PSP-D; SFTP4; SP-D; collectin 7; pulmonary surfactant apoprotein; pulmonary surfactant-associated protein D; surfactant; pulmonary-associated protein D; surfactant-associated protein; pulmonary

Product Properties

Kit components • Human Pulmonary surfactant-associated protein D microplate
• Human Pulmonary surfactant-associated protein D standard
• Human Pulmonary surfactant-associated protein D detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Pulmonary surfactant-associated protein D (SP-D) ELISA Kit has high sensitivity and excellent specificity for detection of Human SFTPD. No significant cross-reactivity or interference between Human SFTPD and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Surfactant protein D (SP-D) belongs to the collectin family.These proteins are oligomeric proteins composed of carbohydrate-recognition domains(CRD)attached to collagenous regions.Collectins are structurally similar to the ficolins although they make use of different CRD structures:C-type lectin domain for the collectins.The lung is the major site of synthesis of SP-D, where the molecules are produced and secreted onto the epithelial surface by alveolar type Ⅱcells and unciliated bronchial epithelial cells. SP-D is also found in different epithelial cells of the gastrointestinal tract and in epithelial cells of various exocrine glands. SP-D is an important factor in the pulmonary anti-microbial defense. The anti-microbial defense mechanisms of SP-D are direct opsonization, neutralization and agglutination.
Gene ID 6441
Alternative SFTPD; COLEC7; PSP-D; SFTP4; SP-D; collectin 7; pulmonary surfactant apoprotein; pulmonary surfactant-associated protein D; surfactant; pulmonary-associated protein D; surfactant-associated protein; pulmonary
Accession P35247

Image & description

Fig.1. Human Pulmonary surfactant-associated protein D (SP-D) Standard Curve.

Fig.1. Human Pulmonary surfactant-associated protein D (SP-D) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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