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Human Protein-arginine deiminase type-4 antibody (PADI4-Ab) ELISA Kit

Human Protein-arginine deiminase type-4 antibody (PADI4-Ab) ELISA Kit

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Specification

Product name Human Protein-arginine deiminase type-4 antibody (PADI4-Ab) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Protein-arginine deiminase type-4 antibody (PADI4-Ab) ELISA Kit employs a two-site sandwich ELISA to quantitate PADI4-Ab in samples. An antibody specific for PADI4-Ab has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPADI4-Ab present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PADI4-Ab is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PADI4-Ab bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative PADI4-Ab; antibody for PAD; PAD4; PADI5; PDI4; PDI5; HL-60 PAD; PADI-H protein; peptidyl arginine deiminase; type V; protein-arginine deiminase

Product Properties

Kit components • Human Protein-arginine deiminase type-4 antibody microplate
• Human Protein-arginine deiminase type-4 antibody standard
• Human Protein-arginine deiminase type-4 antibody detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Protein-arginine deiminase type-4 antibody (PADI4-Ab) ELISA Kit has high sensitivity and excellent specificity for detection of Human PADI4-Ab. No significant cross-reactivity or interference between Human PADI4-Ab and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background PADI4 is a member of a gene family which encodes enzymes responsible for the conversion of arginine residues to citrulline residues. This gene may play a role in granulocyte and macrophage development leading to inflammation and immune response.The deduced 663-amino acid protein, approximately 50% identical to rat PAD types I, II, and III and 73% identical with rat keratinocyte PAD IV, is most conserved in the C terminal two-thirds of the sequence. Functional analysis indicated that the recombinant protein is fully active against arginine-containing substrates. Western blot analysis showed that expression of a 67-kD protein is induced by incubation of HL60 cells with differentiation factors.
Gene ID 23569
Alternative PADI4-Ab; antibody for PAD; PAD4; PADI5; PDI4; PDI5; HL-60 PAD; PADI-H protein; peptidyl arginine deiminase; type V; protein-arginine deiminase
Accession Q9UM07

Image & description

Fig.1. Human Protein-arginine deiminase type-4 antibody (PADI4-Ab) Standard Curve.

Fig.1. Human Protein-arginine deiminase type-4 antibody (PADI4-Ab) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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