|Product name||Human N-methyl-D-aspartic Acid IgG Antibody (NMDA-Ab-IgG) ELISA Kit|
|Applications notes||This Human N-methyl-D-aspartic Acid IgG Antibody (NMDA-Ab-IgG) ELISA Kit employs the competitive enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to NMDA-Ab-IgG. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated NMDA-Ab-IgG and incubated. The competitive inhibition reaction is launched between with HRP labeled NMDA-Ab-IgG and unlabeled NMDA-Ab-IgG with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of NMDA-Ab-IgG in the sample. The color development is stopped and the intensity of the color is measured.|
|SampleType||Cell culture supernatants, Other biological fluids, Plasma, Serum|
|Assay type||Sandwich ELISA (quantitative)|
|Assay duration||Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.|
|Kit components||• Human N-methyl-D-aspartic Acid IgG Antibody microplate
• Human N-methyl-D-aspartic Acid IgG Antibody standard
• Human N-methyl-D-aspartic Acid IgG Antibody detect antibody
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
|Features & Benefits||Human N-methyl-D-aspartic Acid IgG Antibody (NMDA-Ab-IgG) ELISA Kit has high sensitivity and excellent specificity for detection of Human NMDA-Ab-IgG. No significant cross-reactivity or interference between Human NMDA-Ab-IgG and analogues was observed.|
|Calibration range||Please inquire|
|Limit of detection||Please inquire|
|Usage notes||• Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
|Storage instructions||The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.|
|Shipping||Gel pack with blue ice.|
|Precautions||The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.|
|Background||Glutamate [NMDA] receptor subunit zeta-1 is a critical subunit of N-methyl-D-aspartate receptors, members of the glutamate receptor channel superfamily which are heteromeric protein complexes with multiple subunits arranged to form a ligand-gated ion channel. These subunits play a key role in the plasticity of synapses, which is believed to underlie memory and learning. The gene consists of 21 exons and is alternatively spliced, producing transcript variants differing in the C-terminus. Although the sequence of exon 5 is identical in human and rat, the alternative exon 5 splicing in rat has yet to be demonstrated in human. Cell-specific factors are thought to control expression of different isoforms, possibly contributing to the functional diversity of the subunits.|
Fig.1. Human N-methyl-D-aspartic Acid IgG Antibody (NMDA-Ab-IgG) Standard Curve.
Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.