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Human Muscle Skeletal Receptor Tyrosine Kinase antibody (MUSK-Ab) ELISA Kit

Human Muscle Skeletal Receptor Tyrosine Kinase antibody (MUSK-Ab) ELISA Kit

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Specification

Product name Human Muscle Skeletal Receptor Tyrosine Kinase antibody (MUSK-Ab) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Muscle Skeletal Receptor Tyrosine Kinase antibody (MUSK-Ab) ELISA Kit employs the competitive enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to MUSK-Ab. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated MUSK-Ab and incubated. The competitive inhibition reaction is launched between with HRP labeled MUSK-Ab and unlabeled MUSK-Ab with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of MUSK-Ab in the sample. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative MUSK-Ab

Product Properties

Kit components • Human Muscle Skeletal Receptor Tyrosine Kinase antibody microplate
• Human Muscle Skeletal Receptor Tyrosine Kinase antibody standard
• Human Muscle Skeletal Receptor Tyrosine Kinase antibody detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Muscle Skeletal Receptor Tyrosine Kinase antibody (MUSK-Ab) ELISA Kit has high sensitivity and excellent specificity for detection of Human MUSK-Ab. No significant cross-reactivity or interference between Human MUSK-Ab and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background MuSK (for Muscle Specific Kinase) is a receptor tyrosine kinase required for the formation of the neuromuscular junction. It is activated by a nerve-derived proteoglycan called agrin. This protein binds to several receptors on the surface of skeletal muscle. The receptor which seems to be required for formation of the neuromuscular junction (NMJ), which comprises the nerve-muscle synapse is called MuSK (Muscle specific kinase). MuSK is a receptor tyrosine kinase - meaning that it induces cellular signaling by causing the addition of phosphate molecules to particular tyrosines on itself, and on proteins which bind the cytoplasmic domain of the receptor.In mice which are deficient for either agrin or MuSK, the neuromuscular junction does not form. Many other proteins also comprise the NMJ, and are required to maintain its integrity.
Alternative MUSK-Ab

Image & description

Fig.1. Human Muscle Skeletal Receptor Tyrosine Kinase antibody (MUSK-Ab) Standard Curve.

Fig.1. Human Muscle Skeletal Receptor Tyrosine Kinase antibody (MUSK-Ab) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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